BMRB Entry 27759

Name: 1H and 15N Chemical assignment for NSL2-pep of BRMS1
Published: 2019-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27759

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical assignment for NSL2-pep of BRMS1

Deposition date:

2019-01-21

Original release date:

2019-02-13

Authors:

Neira, Jose Luis; Pantoja-Uceda, David

Citation:

Citation: Pantoja-Uceda, David; Neira, Jose Luis; Contreras, Lellys; Manton, Christa; Welch, Danny; Rizzuti, Bruno. "The isolated C-terminal nuclear localization sequence of the breast cancer metastasis suppressor 1 is disordered" Arch. Biochem. Biophys. 664, 95-101 (2019).
PubMed: 30707944

Assembly members:

Assembly members:
NLS2, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NLS2: KARAAVSPQKRKSDGP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	14
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NLS2	1

Entities:

Entity 1, NLS2 16 residues - Formula weight is not available

1

LYS

ALA

ARG

ALA

VAL

SER

PRO

GLN

LYS

2

ARG

LYS

SER

ASP

GLY

PRO

Samples:

sample_1: NLS2 1.8 mM; ammonium acetate 50 mM; TSP 1%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H ROESY	sample_1	isotropic	sample_conditions_1
DOSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks