BMRB Entry 27757

Name: Backbone assignment of SQT-1N
Published: 2019-06-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27757

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of SQT-1N

Deposition date:

2019-01-17

Original release date:

2019-06-27

Authors:

Zalar, Matja; Indrakumar, Sowmya; Levy, Colin; Tunnicliffe, Richard; Peters, G nther; Golovanov, Alexander

Citation:

Citation: Zalar, Matja; Indrakumar, Sowmya; Levy, Colin; Tunnicliffe, Richard; Peters, Gunther; Golovanov, Alexander. "Studies of the oligomerisation mechanism of a cystatin-based engineered protein scaffold." Sci. Rep. 9, 9067-9067 (2019).
PubMed: 31227800

Assembly members:

Assembly members:
SQT-1N, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SQT-1N: MGSSHHHHHHSSGLVPRGSM IPRGLSEAKPATPEIQEIVD KVKPQLEEKTNETYGKLEAV QYKTQVLDTYRYILASTNYY IKVRAGDNKYMHLKVFNGPE QKLISEEDLADRVLTGYQVD KNKDDELTGF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	290
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SQT-1N monomer	1

Entities:

Entity 1, SQT-1N monomer 130 residues - Formula weight is not available

Residues 1-19 represent a non-native affinity tag. Residues 68-73 represent the inserted AU1 peptide. Residues 100-109 represent the inserted c-Myc peptide.

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	MET
3	ILE	PRO	ARG	GLY	LEU	SER	GLU	ALA	LYS	PRO
4	ALA	THR	PRO	GLU	ILE	GLN	GLU	ILE	VAL	ASP
5	LYS	VAL	LYS	PRO	GLN	LEU	GLU	GLU	LYS	THR
6	ASN	GLU	THR	TYR	GLY	LYS	LEU	GLU	ALA	VAL
7	GLN	TYR	LYS	THR	GLN	VAL	LEU	ASP	THR	TYR
8	ARG	TYR	ILE	LEU	ALA	SER	THR	ASN	TYR	TYR
9	ILE	LYS	VAL	ARG	ALA	GLY	ASP	ASN	LYS	TYR
10	MET	HIS	LEU	LYS	VAL	PHE	ASN	GLY	PRO	GLU
11	GLN	LYS	LEU	ILE	SER	GLU	GLU	ASP	LEU	ALA
12	ASP	ARG	VAL	LEU	THR	GLY	TYR	GLN	VAL	ASP
13	LYS	ASN	LYS	ASP	ASP	GLU	LEU	THR	GLY	PHE

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 27757

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	MET
3	ILE	PRO	ARG	GLY	LEU	SER	GLU	ALA	LYS	PRO
4	ALA	THR	PRO	GLU	ILE	GLN	GLU	ILE	VAL	ASP
5	LYS	VAL	LYS	PRO	GLN	LEU	GLU	GLU	LYS	THR
6	ASN	GLU	THR	TYR	GLY	LYS	LEU	GLU	ALA	VAL
7	GLN	TYR	LYS	THR	GLN	VAL	LEU	ASP	THR	TYR
8	ARG	TYR	ILE	LEU	ALA	SER	THR	ASN	TYR	TYR
9	ILE	LYS	VAL	ARG	ALA	GLY	ASP	ASN	LYS	TYR
10	MET	HIS	LEU	LYS	VAL	PHE	ASN	GLY	PRO	GLU
11	GLN	LYS	LEU	ILE	SER	GLU	GLU	ASP	LEU	ALA
12	ASP	ARG	VAL	LEU	THR	GLY	TYR	GLN	VAL	ASP
13	LYS	ASN	LYS	ASP	ASP	GLU	LEU	THR	GLY	PHE

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	MET
3	ILE	PRO	ARG	GLY	LEU	SER	GLU	ALA	LYS	PRO
4	ALA	THR	PRO	GLU	ILE	GLN	GLU	ILE	VAL	ASP
5	LYS	VAL	LYS	PRO	GLN	LEU	GLU	GLU	LYS	THR
6	ASN	GLU	THR	TYR	GLY	LYS	LEU	GLU	ALA	VAL
7	GLN	TYR	LYS	THR	GLN	VAL	LEU	ASP	THR	TYR
8	ARG	TYR	ILE	LEU	ALA	SER	THR	ASN	TYR	TYR
9	ILE	LYS	VAL	ARG	ALA	GLY	ASP	ASN	LYS	TYR
10	MET	HIS	LEU	LYS	VAL	PHE	ASN	GLY	PRO	GLU
11	GLN	LYS	LEU	ILE	SER	GLU	GLU	ASP	LEU	ALA
12	ASP	ARG	VAL	LEU	THR	GLY	TYR	GLN	VAL	ASP
13	LYS	ASN	LYS	ASP	ASP	GLU	LEU	THR	GLY	PHE

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	MET
3	ILE	PRO	ARG	GLY	LEU	SER	GLU	ALA	LYS	PRO
4	ALA	THR	PRO	GLU	ILE	GLN	GLU	ILE	VAL	ASP
5	LYS	VAL	LYS	PRO	GLN	LEU	GLU	GLU	LYS	THR
6	ASN	GLU	THR	TYR	GLY	LYS	LEU	GLU	ALA	VAL
7	GLN	TYR	LYS	THR	GLN	VAL	LEU	ASP	THR	TYR
8	ARG	TYR	ILE	LEU	ALA	SER	THR	ASN	TYR	TYR
9	ILE	LYS	VAL	ARG	ALA	GLY	ASP	ASN	LYS	TYR
10	MET	HIS	LEU	LYS	VAL	PHE	ASN	GLY	PRO	GLU
11	GLN	LYS	LEU	ILE	SER	GLU	GLU	ASP	LEU	ALA
12	ASP	ARG	VAL	LEU	THR	GLY	TYR	GLN	VAL	ASP
13	LYS	ASN	LYS	ASP	ASP	GLU	LEU	THR	GLY	PHE