BMRB Entry 27747

Name: Assignment of the ARC4 domain of human Tankyrase
Published: 2019-04-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27747

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the ARC4 domain of human Tankyrase

Deposition date:

2019-01-07

Original release date:

2019-04-12

Authors:

Pfuhl, Mark; Guettler, Sebastian; Zaleska, Mariola

Citation:

Citation: Zaleska, Mariola; Pollock, Katie; Collins, Ian; Guettler, Sebastian; Pfuhl, Mark. "Assignment of the ARC4 domain of human Tankyrase" Biomol. NMR Assign. 13, 255-260 (2019).
PubMed: 30847846

Assembly members:

Assembly members:
ARC4, polymer, 165 residues, 17830 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ARC4: GAMGNSEADRQLLEAAKAGD VETVKKLCTVQSVNCRDIEG RQSTPLHFAAGYNRVSVVEY LLQHGADVHAKDKGGLVPLH NACSYGHYEVAELLVKHGAV VNVADLWKFTPLHEAAAKGK YEICKLLLQHGADPTKKNRD GNTPLDLVKDGDTDIQDLLR GDAAL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	174
1H chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ARC4 domain of human Tankyrase	1

Entities:

Entity 1, ARC4 domain of human Tankyrase 165 residues - 17830 Da.

the first three residues are a cloning artifact

1

GLY

ALA

MET

GLY

ASN

SER

GLU

ALA

ASP

ARG

2

GLN

LEU

GLU

ALA

LYS

ALA

GLY

ASP

3

VAL

GLU

THR

VAL

LYS

LEU

CYS

THR

VAL

4

GLN

SER

VAL

ASN

CYS

ARG

ASP

ILE

GLU

GLY

5

ARG

GLN

SER

THR

PRO

LEU

HIS

PHE

ALA

6

GLY

TYR

ASN

ARG

VAL

SER

VAL

GLU

TYR

7

LEU

GLN

HIS

GLY

ALA

ASP

VAL

HIS

ALA

8

LYS

ASP

LYS

GLY

LEU

VAL

PRO

LEU

HIS

9

ASN

ALA

CYS

SER

TYR

GLY

HIS

TYR

GLU

VAL

10

ALA

GLU

LEU

VAL

LYS

HIS

GLY

ALA

VAL

11

VAL

ASN

VAL

ALA

ASP

LEU

TRP

LYS

PHE

THR

12

PRO

LEU

HIS

GLU

ALA

LYS

GLY

LYS

13

TYR

GLU

ILE

CYS

LYS

LEU

GLN

HIS

14

GLY

ALA

ASP

PRO

THR

LYS

ASN

ARG

ASP

15

GLY

ASN

THR

PRO

LEU

ASP

LEU

VAL

LYS

ASP

16

GLY

ASP

THR

ASP

ILE

GLN

ASP

LEU

ARG

17

GLY

ASP

ALA

LEU

Samples:

sample_1: ARC4, [U-98% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: ARC4, [U-95% 13C; U-95% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D (H)C(CCO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CCPnmr v2.4, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks