BMRB Entry 27737

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27737

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Title:
15N, 13C and 1H backbone resonance assignments of FKBP12 protein from the pathogenic fungi Mucor circinelloides bound to FK506
Deposition date:
2018-12-20
Original release date:
2019-03-08
Authors:
Gobeil, Sophie; Bobay, Benjamin; Spicer, Leonard; Venters, Ronald
Citation:

Citation: Gobeil, Sophie; Bobay, Benjamin; Spicer, Leonard; Venters, Ronald. "15N, 13C and 1H resonance assignments of FKBP12 proteins from the pathogenic fungi Mucor circinelloides and Aspergillus fumigatus"  Biomol. NMR Assignments 13, 207-212 (2019).
PubMed: 30707421

Assembly members:

Assembly members:
FKBP12, polymer, 108 residues, Formula weight is not available
8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN, non-polymer, 804.018 Da.

Natural source:

Natural source:   Common Name: Mucor circinelloides   Taxonomy ID: 36080   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Mucor circinelloides

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FKBP12: MGVTVERIAPGDGKNFPKKG DKVTIHYVGTLENGDKFDSS RDRGSPFQCTIGVGQVIKGW DEGVTQLSVGEKARLICTHD YAYGERGYPGLIPPKATLNF EVELIKIN

Data sets:
Data typeCount
13C chemical shifts353
15N chemical shifts100
1H chemical shifts438

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FKBP121
2Tacrolimus2

Entities:

Entity 1, FKBP12 108 residues - Formula weight is not available

1   METGLYVALTHRVALGLUARGILEALAPRO
2   GLYASPGLYLYSASNPHEPROLYSLYSGLY
3   ASPLYSVALTHRILEHISTYRVALGLYTHR
4   LEUGLUASNGLYASPLYSPHEASPSERSER
5   ARGASPARGGLYSERPROPHEGLNCYSTHR
6   ILEGLYVALGLYGLNVALILELYSGLYTRP
7   ASPGLUGLYVALTHRGLNLEUSERVALGLY
8   GLULYSALAARGLEUILECYSTHRHISASP
9   TYRALATYRGLYGLUARGGLYTYRPROGLY
10   LEUILEPROPROLYSALATHRLEUASNPHE
11   GLUVALGLULEUILELYSILEASN

Entity 2, Tacrolimus - C44 H69 N O12 - 804.018 Da.

1   FK5

Related Database Links:

NCBI AGW24132.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks