BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27729

Title: IL37 53-206 dimer   PubMed: 30819901

Deposition date: 2018-12-15 Original release date: 2019-01-15

Authors: Eisenmesser, Elan

Citation: Eisenmesser, Elan; Gottschlich, Adrian; Redzic, Jasmina; Paukovich, Natasia; Nix, Jay; Azam, Tania; Zhang, Lingdi; Zhao, Rui; Kieft, Jeffrey; The, Erlinda; Meng, Xianzhong; Dinarello, Charles. "Interleukin-37 monomer is the active form for reducing innate immunity."  Proc. Natl. Acad. Sci. U.S.A. 116, 5514-5522 (2019).

Assembly members:
IL37_dimer, polymer, 218 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):
IL37_dimer: MSFVGENSGVKMGSEDWEKD EPQCCLEDPAGSPLEPGPSL PTMNFVHTSPKVKNLNPKKF SIHDQDHKVLVLDSGNLIAV PDKNYIRPEIFFALASSLSS ASAEKGSPILLGVSKGEFCL YCDKDKGQSHPSLQLKKEKL MKLAAQKESARRPFIFYRAQ VGSWNMLESAAHPGWFICTS CNCNEPVGVTDKFENRKHIE FSFQPVCKAEMSPSEVSD

Data sets:
Data typeCount
13C chemical shifts444
15N chemical shifts145
1H chemical shifts249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Il37 chain A1
2Il37 chain B1

Entities:

Entity 1, Il37 chain A 218 residues - Formula weight is not available

1   METSERPHEVALGLYGLUASNSERGLYVAL
2   LYSMETGLYSERGLUASPTRPGLULYSASP
3   GLUPROGLNCYSCYSLEUGLUASPPROALA
4   GLYSERPROLEUGLUPROGLYPROSERLEU
5   PROTHRMETASNPHEVALHISTHRSERPRO
6   LYSVALLYSASNLEUASNPROLYSLYSPHE
7   SERILEHISASPGLNASPHISLYSVALLEU
8   VALLEUASPSERGLYASNLEUILEALAVAL
9   PROASPLYSASNTYRILEARGPROGLUILE
10   PHEPHEALALEUALASERSERLEUSERSER
11   ALASERALAGLULYSGLYSERPROILELEU
12   LEUGLYVALSERLYSGLYGLUPHECYSLEU
13   TYRCYSASPLYSASPLYSGLYGLNSERHIS
14   PROSERLEUGLNLEULYSLYSGLULYSLEU
15   METLYSLEUALAALAGLNLYSGLUSERALA
16   ARGARGPROPHEILEPHETYRARGALAGLN
17   VALGLYSERTRPASNMETLEUGLUSERALA
18   ALAHISPROGLYTRPPHEILECYSTHRSER
19   CYSASNCYSASNGLUPROVALGLYVALTHR
20   ASPLYSPHEGLUASNARGLYSHISILEGLU
21   PHESERPHEGLNPROVALCYSLYSALAGLU
22   METSERPROSERGLUVALSERASP

Samples:

sample_1: IL37 dimer, [U-100% 15N], 1 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM

sample_2: IL37 dimer, [U-13C; U-15N; U-2H], 1 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts