BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27726

Title: Arf1_L8K_GTPgS   PubMed: 30735034

Deposition date: 2018-12-12 Original release date: 2019-04-12

Authors: Li, Yifei; Li, Jess; Soubias, Olivier; Sun, Shangjin; Randazzo, Paul; Byrd, R. Andrew

Citation: Li, Yifei; Soubias, Olivier; Li, Jess; Sun, Shangjin; Randazzo, Paul; Byrd, R Andrew. "Functional Expression and Characterization of Human Myristoylated-Arf1 in Nanodisc Membrane Mimetics."  Biochemistry 58, 1423-1431 (2019).

Assembly members:
Arf1_L8K, polymer, 181 residues, 20711.78 Da.
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, non-polymer, 539.246 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET19b

Entity Sequences (FASTA):
Arf1_L8K: MGNIFANKFKGLFGKKEMRI LMVGLDAAGKTTILYKLKLG EIVTTIPTIGFNVETVEYKN ISFTVWDVGGQDKIRPLWRH YFQNTQGLIFVVDSNDRERV NEAREELMRMLAEDELRDAV LLVFANKQDLPNAMNAAEIT DKLGLHSLRHRNWYIQATCA TSGDGLYEGLDWLSNQLRNQ K

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts130
1H chemical shifts355

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Arf1_L8K1
2GTPgS2

Entities:

Entity 1, Arf1_L8K 181 residues - 20711.78 Da.

1   METGLYASNILEPHEALAASNLYSPHELYS
2   GLYLEUPHEGLYLYSLYSGLUMETARGILE
3   LEUMETVALGLYLEUASPALAALAGLYLYS
4   THRTHRILELEUTYRLYSLEULYSLEUGLY
5   GLUILEVALTHRTHRILEPROTHRILEGLY
6   PHEASNVALGLUTHRVALGLUTYRLYSASN
7   ILESERPHETHRVALTRPASPVALGLYGLY
8   GLNASPLYSILEARGPROLEUTRPARGHIS
9   TYRPHEGLNASNTHRGLNGLYLEUILEPHE
10   VALVALASPSERASNASPARGGLUARGVAL
11   ASNGLUALAARGGLUGLULEUMETARGMET
12   LEUALAGLUASPGLULEUARGASPALAVAL
13   LEULEUVALPHEALAASNLYSGLNASPLEU
14   PROASNALAMETASNALAALAGLUILETHR
15   ASPLYSLEUGLYLEUHISSERLEUARGHIS
16   ARGASNTRPTYRILEGLNALATHRCYSALA
17   THRSERGLYASPGLYLEUTYRGLUGLYLEU
18   ASPTRPLEUSERASNGLNLEUARGASNGLN
19   LYS

Entity 2, GTPgS - C10 H16 N5 O13 P3 S - 539.246 Da.

1   GSP

Samples:

sample_1: Arf1_L8K_GTPgS, [U-100% 13C; U-100% 15N; U-95% 2H] ILV methyl protonated, 500 uM

sample_2: Arf1_L8K_GTPgS, [U-100% 15N; U-95% 2H] ILV methyl 13C labeled and protonated, 500 uM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
4D methyl-methyl NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, data analysis, peak picking, processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 850 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts