BMRB Entry 27724

Name: NMR Assignments of the apo-form of Aedes aegypti odorant binding protein 22
Published: 2018-12-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27724

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignments of the apo-form of Aedes aegypti odorant binding protein 22

Deposition date:

2018-12-11

Original release date:

2018-12-19

Authors:

Jones, David; Wang, Jing

Citation:

Citation: Wang, Jing; Murphy, Emma; Nix, Jay; Jones, David. "Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail" Sci. Rep. 10, 3300-3300 (2020).
PubMed: 32094450

Assembly members:

Assembly members:
OBP22, polymer, 123 residues, 14379.2591 Da.

Natural source:

Natural source: Common Name: Yellow Fever Mosquito Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Aedes aegypti

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Aedes aegypti Vector: pET13a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OBP22: MEFTVSTTEDLQRYRTECVS SLNIPADYVEKFKKWEFPED DTTMCYIKCVFNKMQLFDDT EGPLVDNLVHQLAHGRDAEE VRTEVLKCVDKNTDNNACHW AFRGFKCFQKNNLSLIKASI KKD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	241
15N chemical shifts	128
1H chemical shifts	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Aedes aegypti OBP22	1

Entities:

Entity 1, Aedes aegypti OBP22 123 residues - 14379.2591 Da.

Residue 1 is initiator methionine. This fragment represent the mature processed protein without the signal peptide.

1

MET

GLU

PHE

THR

VAL

SER

THR

GLU

ASP

2

LEU

GLN

ARG

TYR

ARG

THR

GLU

CYS

VAL

SER

3

SER

LEU

ASN

ILE

PRO

ALA

ASP

TYR

VAL

GLU

4

LYS

PHE

LYS

TRP

GLU

PHE

PRO

GLU

ASP

5

ASP

THR

MET

CYS

TYR

ILE

LYS

CYS

VAL

6

PHE

ASN

LYS

MET

GLN

LEU

PHE

ASP

THR

7

GLU

GLY

PRO

LEU

VAL

ASP

ASN

LEU

VAL

HIS

8

GLN

LEU

ALA

HIS

GLY

ARG

ASP

ALA

GLU

9

VAL

ARG

THR

GLU

VAL

LEU

LYS

CYS

VAL

ASP

10

LYS

ASN

THR

ASP

ASN

ALA

CYS

HIS

TRP

11

ALA

PHE

ARG

GLY

PHE

LYS

CYS

PHE

GLN

LYS

12

ASN

LEU

SER

LEU

ILE

LYS

ALA

SER

ILE

13

LYS

ASP

Samples:

H2O: Mol2, [U-95% 13C; U-95% 15N], 0.65 ± 1e-05 mM; sodium phosphate 20 mM

CondSet1: ionic strength: 20.000 mM; pH: 6.500; pressure: 820.000 mbar; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	H2O	isotropic	CondSet1
3D HNCACB	H2O	isotropic	CondSet1
APO_CBCACONH (H[N[co[{CA\|ca[C]}]]])	H2O	isotropic	CondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - Peal Picking

nmrDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection

IstHMS vv211, Hyberts, Milbrandt, Wagner, Haribabu & Wagner - collection, processing

NMR spectrometers:

Varian UnityInova 600 MHz

UniProt	Q1HRL7
AlphaFold	Q1HRL7