BMRB Entry 27717

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27717

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Title:
Androgen Receptor (AR) polyQ-derived peptide L4Q20
Deposition date:
2018-12-05
Original release date:
2019-06-13
Authors:
Salvatella, Xavier; Escobedo, Albert
Citation:

Citation: Escobedo, Albert; Topal, Busra; Kunze, Micha; Aranda, Juan; Chiesa, Giulio; Mungianu, Daniele; Bernardo-Seisdedos, Ganeko; Eftekharzadeh, Bahareh; Gairi, Margarida; Pierattelli, Roberta; Felli, Isabella; Diercks, Tammo; Millet, Oscar; Garcia, Jesus; Orozco, Modesto; Crehuet, Ramon; Lindorff-Larsen, Kresten; Salvatella, Xavier. "Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor."  Nat. Commun. 10, 2034-2034 (2019).
PubMed: 31048691

Assembly members:

Assembly members:
L4Q20, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L4Q20: KKPGASLLLLQQQQQQQQQQ QQQQQQQQQQKK

Data sets:
Data typeCount
13C chemical shifts75
15N chemical shifts30
1H chemical shifts30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L4Q201

Entities:

Entity 1, L4Q20 32 residues - Formula weight is not available

1   LYSLYSPROGLYALASERLEULEULEULEU
2   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
4   LYSLYS

Samples:

sample_1: L4Q20, [U-95% 13C; U-95% 15N], 100 uM; DSS 10 uM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.09 M; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr_Analysis, CCPN - chemical shift assignment

TopSpin, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks