BMRB Entry 27707

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27707

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Title:
Domain 4 of Suilysin
Deposition date:
2018-11-28
Original release date:
2019-02-22
Authors:
Jen, Freda; Haselhorst, Thomas
Citation:

Citation: Shewell, Lucy; Day, Christopher; Reijneveld, Josephine; Jen, Freda; Haselhorst, Thomas; Atack, John; Lawrence, Sara; Parker, Michael; Brouwer, Stephan; Gillen, Christine; Kobe, Boston; Nizet, Victor; von Itztein, Mark; Walker, Mark; Paton, Adrienne; Paton, James; Jennings, Michael. "All major cholesterol-dependent cytolysins use glycans as cellular receptors."  .

Assembly members:

Assembly members:
Streptococcus_suis_suilysin_doamin_4, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1307   Superkingdom: bacteria   Kingdom: terrabacteria   Genus/species: Streptococcus suis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Streptococcus_suis_suilysin_doamin_4: MSALTLDHSGAYVAKYNITW EEVSYNEAGEEVWEPKAWDK NGVNLTSHWSETIQIPGNAR NLHVNIQECTGLAWEWWRTV YDKDLPLVGQRKITIWGTTL YPQYADEVIE

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts102
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1doamin 41

Entities:

Entity 1, doamin 4 110 residues - Formula weight is not available

1   METSERALALEUTHRLEUASPHISSERGLY
2   ALATYRVALALALYSTYRASNILETHRTRP
3   GLUGLUVALSERTYRASNGLUALAGLYGLU
4   GLUVALTRPGLUPROLYSALATRPASPLYS
5   ASNGLYVALASNLEUTHRSERHISTRPSER
6   GLUTHRILEGLNILEPROGLYASNALAARG
7   ASNLEUHISVALASNILEGLNGLUCYSTHR
8   GLYLEUALATRPGLUTRPTRPARGTHRVAL
9   TYRASPLYSASPLEUPROLEUVALGLYGLN
10   ARGLYSILETHRILETRPGLYTHRTHRLEU
11   TYRPROGLNTYRALAASPGLUVALILEGLU

Samples:

sample_1: Streptococcus suis suilysin doamin 4, [U-100% 13C; U-100% 15N], 0.5mM mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - data analysis

NMR spectrometers:

  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks