BMRB Entry 27689

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Vibrio cholerae DciA 1-111
Published: 2021-05-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27689

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Vibrio cholerae DciA 1-111

Deposition date:

2018-11-12

Original release date:

2021-05-19

Authors:

Andreani, Jessica; Ochsenbein, Francoise; Quevillon-Cheruel, Sophie

Citation:

Citation: Marsin, Stephanie; Adam, Yazid; Cargemel, Claire; Andreani, Jessica; Baconnais, Sonia; Legrand, Pierre; Gallay-Li de la Sierra, Ines; Humbert, Adeline; Aumont-Nicaise, Magali; Velours, Christophe; Ochsenbein, Francoise; Durand, Dominique; Le Cam, Eric; Walbott, Helene; Possoz, Christophe; Quevillon-Cheruel, Sophie; Ferat, Jean-Luc. "Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase" Nucleic Acids Res. 49, 6569-6586 (2021).
PubMed: 34107018

Assembly members:

Assembly members:
DciA, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: V. cholerae Taxonomy ID: 666 Superkingdom: Bacteria Kingdom: Eubacteria Genus/species: Vibrio cholerae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DciA: MHHHHHHRDHRPTATDELIQ ASKLKQIQEHAKAILLINRQ LQDILPKGLKTQVRAANVRG GNLVLEAASAALKMKVDYER LHILTQLRQNGFGHLISIEV RVNPELYRQSKITSEDA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	107
1H chemical shifts	368

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DciA monomer	1

Entities:

Entity 1, DciA monomer 117 residues - Formula weight is not available

1

MET

HIS

ARG

ASP

HIS

2

ARG

PRO

THR

ALA

THR

ASP

GLU

LEU

ILE

GLN

3

ALA

SER

LYS

LEU

LYS

GLN

ILE

GLN

GLU

HIS

4

ALA

LYS

ALA

ILE

LEU

ILE

ASN

ARG

GLN

5

LEU

GLN

ASP

ILE

LEU

PRO

LYS

GLY

LEU

LYS

6

THR

GLN

VAL

ARG

ALA

ASN

VAL

ARG

GLY

7

GLY

ASN

LEU

VAL

LEU

GLU

ALA

SER

ALA

8

ALA

LEU

LYS

MET

LYS

VAL

ASP

TYR

GLU

ARG

9

LEU

HIS

ILE

LEU

THR

GLN

LEU

ARG

GLN

ASN

10

GLY

PHE

GLY

HIS

LEU

ILE

SER

ILE

GLU

VAL

11

ARG

VAL

ASN

PRO

GLU

LEU

TYR

ARG

GLN

SER

12

LYS

ILE

THR

SER

GLU

ASP

ALA

Samples:

sample_1: DciA, [U-100% 13C; U-100% 15N], 0.9 mM; DSS 0.2 mM; sodium azide 0.1 mg/mL; EDTA 2 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N SOFAST HMQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks