BMRB Entry 27688

Name: Chemical Shift Assignments for Interleukin-36beta isoform-2
Published: 2019-04-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27688

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments for Interleukin-36beta isoform-2

Deposition date:

2018-11-09

Original release date:

2019-04-25

Authors:

Ohlenschlaeger, Oliver; Kumar, Amit

Citation:

Citation: Kumar, Amit; Wissbrock, Amelie; Bellstedt, Peter; Lang, Andras; Ramachandran, Ramadurai; Wiedemann, Christoph; Imhof, Diana; Ohlenschlaeger, Oliver. "(1)H, (13)C, and (15)N resonance assignments of the cytokine interleukin-36beta isoform-2" Biomol. NMR Assignments 13, 155-161 (2019).
PubMed: 30758717

Assembly members:

Assembly members:
IL36B2, polymer, 153 residues, 17231.86 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-45b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IL36B2: REAAPKSYAIRDSRQMVWVL SGNSLIAAPLSRSIKPVTLH LIACRDTEFSDKEKGNMVYL GIKGKDLCLFCAEIQGKPTL QLKEKNIMDLYVEKKAQKPF LFFHNKEGSTSVFQSVSYPG WFIATSTTSGQPIFLTKERG ITNNTNFYLDSVE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	646
15N chemical shifts	156
1H chemical shifts	985

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IL36B2	1

Entities:

Entity 1, IL36B2 153 residues - 17231.86 Da.

1

ARG

GLU

ALA

PRO

LYS

SER

TYR

ALA

ILE

2

ARG

ASP

SER

ARG

GLN

MET

VAL

TRP

VAL

LEU

3

SER

GLY

ASN

SER

LEU

ILE

ALA

PRO

LEU

4

SER

ARG

SER

ILE

LYS

PRO

VAL

THR

LEU

HIS

5

LEU

ILE

ALA

CYS

ARG

ASP

THR

GLU

PHE

SER

6

ASP

LYS

GLU

LYS

GLY

ASN

MET

VAL

TYR

LEU

7

GLY

ILE

LYS

GLY

LYS

ASP

LEU

CYS

LEU

PHE

8

CYS

ALA

GLU

ILE

GLN

GLY

LYS

PRO

THR

LEU

9

GLN

LEU

LYS

GLU

LYS

ASN

ILE

MET

ASP

LEU

10

TYR

VAL

GLU

LYS

ALA

GLN

LYS

PRO

PHE

11

LEU

PHE

HIS

ASN

LYS

GLU

GLY

SER

THR

12

SER

VAL

PHE

GLN

SER

VAL

SER

TYR

PRO

GLY

13

TRP

PHE

ILE

ALA

THR

SER

THR

SER

GLY

14

GLN

PRO

ILE

PHE

LEU

THR

LYS

GLU

ARG

GLY

15

ILE

THR

ASN

THR

ASN

PHE

TYR

LEU

ASP

16

SER

VAL

GLU

Samples:

sample_1: IL36B2, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM

sample_2: IL36B2, [U-100% 15N], 1 mM; sodium phosphate 20 mM

sample_3: IL36B2, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection, processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

UNP	Q9NZH7
AlphaFold	Q9UHA5