BMRB Entry 27677

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27677

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Title:
Resonance Assignments for the PWWP-ARID domain of human RBBP1
Deposition date:
2018-11-05
Original release date:
2019-02-06
Authors:
Gong, Weibin; Yao, Xingzhe; Liang, Qihui; Tong, Yufeng; Feng, Yingang; Perrett, Sarah
Citation:

Citation: Gong, Weibin; Yao, Xingzhe; Liang, Qihui; Tong, Yufeng; Perrett, Sarah; Feng, Yingang. "Resonance assignments for the tandem PWWP-ARID domains of human RBBP1"  Biomol. NMR Assignments 13, 177-181 (2019).
PubMed: 30666492

Assembly members:

Assembly members:
RBBP1_PWWP-ARID_domain, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBBP1_PWWP-ARID_domain: NDELLGKVVSVVSATERTEW YPALVISPSCNDDITVKKDQ CLVRSFIDSKFYSIARKDIK EVDILNLPESELSTKPGLQK ASIFLKTRVVPDNWKMDISE ILESSSSKDKEKELDPEERD NFLQQLYKFMEDRGTPINKP PVLGYKDLNLFKLFRLVYHQ GGCDNIDSGAVWKQIYMDLG IPILNSAASYNVKTAYRKYL YGFEEYCRSANIQFRTVHHH ELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts906
15N chemical shifts225
1H chemical shifts1481

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBBP1 PWWP-ARID domain1

Entities:

Entity 1, RBBP1 PWWP-ARID domain 229 residues - Formula weight is not available

1   ASNASPGLULEULEUGLYLYSVALVALSER
2   VALVALSERALATHRGLUARGTHRGLUTRP
3   TYRPROALALEUVALILESERPROSERCYS
4   ASNASPASPILETHRVALLYSLYSASPGLN
5   CYSLEUVALARGSERPHEILEASPSERLYS
6   PHETYRSERILEALAARGLYSASPILELYS
7   GLUVALASPILELEUASNLEUPROGLUSER
8   GLULEUSERTHRLYSPROGLYLEUGLNLYS
9   ALASERILEPHELEULYSTHRARGVALVAL
10   PROASPASNTRPLYSMETASPILESERGLU
11   ILELEUGLUSERSERSERSERLYSASPLYS
12   GLULYSGLULEUASPPROGLUGLUARGASP
13   ASNPHELEUGLNGLNLEUTYRLYSPHEMET
14   GLUASPARGGLYTHRPROILEASNLYSPRO
15   PROVALLEUGLYTYRLYSASPLEUASNLEU
16   PHELYSLEUPHEARGLEUVALTYRHISGLN
17   GLYGLYCYSASPASNILEASPSERGLYALA
18   VALTRPLYSGLNILETYRMETASPLEUGLY
19   ILEPROILELEUASNSERALAALASERTYR
20   ASNVALLYSTHRALATYRARGLYSTYRLEU
21   TYRGLYPHEGLUGLUTYRCYSARGSERALA
22   ASNILEGLNPHEARGTHRVALHISHISHIS
23   GLULEUGLUHISHISHISHISHISHIS

Samples:

sample_1: RBBP1 PWWP-ARID domain, [U-100% 13C; U-100% 15N], 0.6-0.8 mM; sodium phosphate 20 mM; sodium chloride 500 mM; DTT 1 mM; EDTA 1 mM; D2O 10%

sample_conditions_1: ionic strength: 540 mM; pH: 6; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks