BMRB Entry 27674

Name: Chemical shifts for C-tail of the apelin receptor in LPPG micelles.
Published: 2018-11-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27674

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts for C-tail of the apelin receptor in LPPG micelles.

Deposition date:

2018-11-02

Original release date:

2018-11-09

Authors:

Rainey, Jan; Pandey, Aditya

Citation:

Citation: Pandey, Aditya; LeBlanc, Danielle; Parmar, Hirendrasinh; Pham, Tran Thanh Tam; Sarker, Muzaddid; Xu, Lingling; Duncan, Roy; Liu, Xiang-Qin; Rainey, Jan. "Structure, amphipathy, and topology of the membrane-proximal helix 8 influence apelin receptor plasma membrane localization" Biochim. Biophys. Acta Biomembr. 1861, 183036-183036 (2019).
PubMed: 31394100

Assembly members:

Assembly members:
AR_ctail, polymer, 71 residues, 7488.1419 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETHN

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AR_ctail: AFFDPRFRQASTSMLCCGQS RSAGTSHSSSGEKSASYSSG HSQGPGPNMGKGGEQMHEKS IPYSQETLVVD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	146
15N chemical shifts	45
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AR ctail	1

Entities:

Entity 1, AR ctail 71 residues - 7488.1419 Da.

1

ALA

PHE

ASP

PRO

ARG

PHE

ARG

GLN

ALA

2

SER

THR

SER

MET

LEU

CYS

GLY

GLN

SER

3

ARG

SER

ALA

GLY

THR

SER

HIS

SER

4

GLY

GLU

LYS

SER

ALA

SER

TYR

SER

GLY

5

HIS

SER

GLN

GLY

PRO

GLY

PRO

ASN

MET

GLY

6

LYS

GLY

GLU

GLN

MET

HIS

GLU

LYS

SER

7

ILE

PRO

TYR

SER

GLN

GLU

THR

LEU

VAL

8

ASP

Samples:

sample_1: AR ctail, [U-13C; U-15N], 0.5 mM; DSS 1 mM; DTT 10 mM; sodium azide 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; LPPG 100 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
HN(CA)CO (H[N[ca[CO]]])	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
CBCACONH (H[N[co[{CA\|ca[C]}]]])	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.2.2, CCPN - data analysis

DANGLE v1.1, CCPN - data analysis

NMRPipe v8.1, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Topspin v3.1, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks