BMRB Entry 27662

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27662

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Title:
amide chemical shifts of human PCNA bound to RecQ5 peptide
Deposition date:
2018-10-25
Original release date:
2019-02-05
Authors:
De Biasio, Alfredo; Blanco, Francisco
Citation:

Citation: Gonzalez-Magana, Amaia; Ibanez de Opakua, Alain; Romano-Moreno, Miguel; Murciano-Calles, Javier; Merino, Nekane; Luque, Irene; Rojas, Adriana; Onesti, Silvia; Blanco, Francisco; De Biasio, Alfredo. "The p12 subunit of human polymerase delta uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA)"  J. Biol. Chem. 294, 3947-3956 (2019).
PubMed: 30655288

Assembly members:

Assembly members:
human_PCNA, polymer, 264 residues, Formula weight is not available
human_RecQ5_peptide_spanning_residues_952-979, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_PCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS
human_RecQ5_peptide_spanning_residues_952-979: KTSPGRSVKEEAQNLIRHFF HGRARCES

Data sets:
Data typeCount
15N chemical shifts218
1H chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1human PCNA1
2RecQ5 peptide2

Entities:

Entity 1, human PCNA 264 residues - Formula weight is not available

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Entity 2, RecQ5 peptide 28 residues - Formula weight is not available

1   LYSTHRSERPROGLYARGSERVALLYSGLU
2   GLUALAGLNASNLEUILEARGHISPHEPHE
3   HISGLYARGALAARGCYSGLUSER

Samples:

sample_1: human PCNA, [U-13C; U-15N; U-2H], 33 uM; human RecQ5 peptide spanning residues 952-979 1208 uM; D2O, [U-2H], 5 v/v; DSS 20 uM; DTT 1 mM; potassium phosphate 20 mM; sodium azide 0.01 % w/v; sodium chloride 140 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks