BMRB Entry 27661

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27661

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Title:
Amide chemical shifts of human PCNA bound to p12 peptide
Deposition date:
2018-10-25
Original release date:
2019-01-29
Authors:
De Biasio, Alfredo; Blanco, Francisco
Citation:

Citation: Gonzalez-Magana, Amaia; Ibanez de Opakua, Alain; Romano-Moreno, Miguel; Murciano-Calles, Javier; Merino, Nekane; Luque, Irene; Rojas, Adriana; Onesti, Silvia; Blanco, Francisco; De Biasio, Alfredo. "The p12 subunit of human polymerase delta uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA)"  J. Biol. Chem. 294, 3947-3956 (2019).
PubMed: 30655288

Assembly members:

Assembly members:
human_PCNA, polymer, 264 residues, Formula weight is not available
p12 peptide, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_PCNA: GPHMFEARLVQGSILKKVLE ALKDLINEACWDISSSGVNL QSMDSSHVSLVQLTLRSEGF DTYRCDRNLAMGVNLTSMSK ILKCAGNEDIITLRAEDNAD TLALVFEAPNQEKVSDYEMK LMDLDVEQLGIPEQEYSCVV KMPSGEFARICRDLSHIGDA VVISCAKDGVKFSASGELGN GNIKLSQTSNVDKEEEAVTI EMNEPVQLTFALRYLNFFTK ATPLSSTVTLSMSADVPLVV EYKIADMGHLKYYLAPKIED EEGS
p12 peptide: MGRKRLITDSYPVVKRREG

Data sets:
Data typeCount
15N chemical shifts202
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PCNA1
2p12 peptide2

Entities:

Entity 1, PCNA 264 residues - Formula weight is not available

1   GLYPROHISMETPHEGLUALAARGLEUVAL
2   GLNGLYSERILELEULYSLYSVALLEUGLU
3   ALALEULYSASPLEUILEASNGLUALACYS
4   TRPASPILESERSERSERGLYVALASNLEU
5   GLNSERMETASPSERSERHISVALSERLEU
6   VALGLNLEUTHRLEUARGSERGLUGLYPHE
7   ASPTHRTYRARGCYSASPARGASNLEUALA
8   METGLYVALASNLEUTHRSERMETSERLYS
9   ILELEULYSCYSALAGLYASNGLUASPILE
10   ILETHRLEUARGALAGLUASPASNALAASP
11   THRLEUALALEUVALPHEGLUALAPROASN
12   GLNGLULYSVALSERASPTYRGLUMETLYS
13   LEUMETASPLEUASPVALGLUGLNLEUGLY
14   ILEPROGLUGLNGLUTYRSERCYSVALVAL
15   LYSMETPROSERGLYGLUPHEALAARGILE
16   CYSARGASPLEUSERHISILEGLYASPALA
17   VALVALILESERCYSALALYSASPGLYVAL
18   LYSPHESERALASERGLYGLULEUGLYASN
19   GLYASNILELYSLEUSERGLNTHRSERASN
20   VALASPLYSGLUGLUGLUALAVALTHRILE
21   GLUMETASNGLUPROVALGLNLEUTHRPHE
22   ALALEUARGTYRLEUASNPHEPHETHRLYS
23   ALATHRPROLEUSERSERTHRVALTHRLEU
24   SERMETSERALAASPVALPROLEUVALVAL
25   GLUTYRLYSILEALAASPMETGLYHISLEU
26   LYSTYRTYRLEUALAPROLYSILEGLUASP
27   GLUGLUGLYSER

Entity 2, p12 peptide 19 residues - Formula weight is not available

1   METGLYARGLYSARGLEUILETHRASPSER
2   TYRPROVALVALLYSARGARGGLUGLY

Samples:

sample_1: human PCNA, [U-13C; U-15N; U-2H], 45.4 uM; human POLD4 (p12) fragment spanning residues 1-19 454 uM; sodium phosphate 10 mM; D2O, [U-2H], 5 % v/v; sodium azide 0.01 % w/v; DSS 20 uM; sodium chloride 140 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks