BMRB Entry 27659

Name: Backbone assignment of human ribonuclease 6
Published: 2018-11-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27659

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of human ribonuclease 6

Deposition date:

2018-10-22

Original release date:

2018-11-28

Authors:

Bernard, David; Gagne, Donald; Letourneau, Myriam; Doucet, Nicolas

Citation:

Citation: Narayanan, Chitra; Bernard, David; Letourneau, Myriam; Gagnon, Jacinthe; Gagne, Donald; Bafna, Khushboo; Calmettes, Charles; Couture, Jean-Francois; Agarwal, Pratul; Doucet, Nicolas. "Insights into Structural and Dynamical Changes Experienced by Human RNase 6 upon Ligand Binding" Biochemistry 59, 755-765 (2020).
PubMed: 31909602

Assembly members:

Assembly members:
RNase6, polymer, 127 residues, 14653 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNase6: WPKRLTKAHWFEIQHIQPSP LQCNRAMSGINNYTQHCKHQ NTFLHDSFQNVAAVCDLLSI VCKNRRHNCHQSSKPVNMTD CRLTSGKYPQCRYSAAAQYK FFIVACDPPQKSDPPYKLVP VHLDSIL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_major_conformer
- assigned_chem_shift_list_minor_conformer

Data type	Count
13C chemical shifts	257
15N chemical shifts	137
1H chemical shifts	146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNase 6, Major_conformer	1
2	RNase 6, Minor_conformer	1

Entities:

Entity 1, RNase 6, Major_conformer 127 residues - 14653 Da.

1

TRP

PRO

LYS

ARG

LEU

THR

LYS

ALA

HIS

TRP

2

PHE

GLU

ILE

GLN

HIS

ILE

GLN

PRO

SER

PRO

3

LEU

GLN

CYS

ASN

ARG

ALA

MET

SER

GLY

ILE

4

ASN

TYR

THR

GLN

HIS

CYS

LYS

HIS

GLN

5

ASN

THR

PHE

LEU

HIS

ASP

SER

PHE

GLN

ASN

6

VAL

ALA

VAL

CYS

ASP

LEU

SER

ILE

7

VAL

CYS

LYS

ASN

ARG

HIS

ASN

CYS

HIS

8

GLN

SER

LYS

PRO

VAL

ASN

MET

THR

ASP

9

CYS

ARG

LEU

THR

SER

GLY

LYS

TYR

PRO

GLN

10

CYS

ARG

TYR

SER

ALA

GLN

TYR

LYS

11

PHE

ILE

VAL

ALA

CYS

ASP

PRO

GLN

12

LYS

SER

ASP

PRO

TYR

LYS

LEU

VAL

PRO

13

VAL

HIS

LEU

ASP

SER

ILE

LEU

Samples:

sample_1: RNase6, [U-99% 13C; U-99% 15N], 680 uM; D2O, [U-99% 2H], 10%; sodium acetate 15 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4, CCPN - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

UNP	Q93091
PDB	4x09 5oab
AlphaFold	Q93091