BMRB Entry 27648

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27648

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for KID domain (C90A, V118D) of CREB
Deposition date:
2018-10-12
Original release date:
2019-01-08
Authors:
Shnitkind, Sergey; Ellis, Jamie; Dyson, H. Jane; Wright, Peter
Citation:

Citation: Shnitkind, Sergey; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Structural basis for graded inhibition of CREB:DNA interactions by multi-site phosphorylation"  Biochemistry 57, 6964-6972 (2018).
PubMed: 30507144

Assembly members:

Assembly members:
KID, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KID: GTVQSSAKDLKRLFSGTQIS TIAESEDSQESVDSDTDSQK RREILSRRPSYRKILNDLSS DAPGVPRIEEEKSEEET

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KID1

Entities:

Entity 1, KID 77 residues - Formula weight is not available

1   GLYTHRVALGLNSERSERALALYSASPLEU
2   LYSARGLEUPHESERGLYTHRGLNILESER
3   THRILEALAGLUSERGLUASPSERGLNGLU
4   SERVALASPSERASPTHRASPSERGLNLYS
5   ARGARGGLUILELEUSERARGARGPROSER
6   TYRARGLYSILELEUASNASPLEUSERSER
7   ASPALAPROGLYVALPROARGILEGLUGLU
8   GLULYSSERGLUGLUGLUTHR

Samples:

sample_1: KID, [U-99% 13C; U-99% 15N], 60 uM; MES 20 mM; sodium chloride 100 mM; H2O 95%; D2O, [U-99% 2H], 5%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker DMX 800 MHz

Related Database Links:

SP P16220
AlphaFold Q9UMA7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks