BMRB Entry 27645

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27645

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Title:
Backbone 1H, 13C and 15N chemical shift assignment of the PDZ domain of the protein tyrosine phosphatase non-receptor type 3 complexed with the HPV16 E6 oncoprotein C-terminal peptide
Deposition date:
2018-10-12
Original release date:
2019-05-15
Authors:
Simenel, Catherine; Samson, Damien; Caillet-Saguy, Celia
Citation:

Citation: Genera, Mariano; Samson, Damien; Raynal, Bertrand; Haouz, Ahmed; Baron, Bruno; Simenel, Catherine; Guerois, Raphael; Wolff, Nicolas; Caillet-Saguy, Celia. "Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein"  Sci. Rep. 9, 7438-7438 (2019).
PubMed: 31092861

Assembly members:

Assembly members:
PDZ_PTPN3, polymer, 94 residues, 10556.05 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ_PTPN3: GDSYLVLIRITPDEDGKFGF NLKGGVDQKMPLVVSRINPE SPADTCIPKLNEGDQIVLIN GRDISEHTHDQVVMFIKASR ESHSRELALVIRRR

Data typeCount
13C chemical shifts254
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ PTPN31

Entities:

Entity 1, PDZ PTPN3 94 residues - 10556.05 Da.

1   GLYASPSERTYRLEUVALLEUILEARGILE
2   THRPROASPGLUASPGLYLYSPHEGLYPHE
3   ASNLEULYSGLYGLYVALASPGLNLYSMET
4   PROLEUVALVALSERARGILEASNPROGLU
5   SERPROALAASPTHRCYSILEPROLYSLEU
6   ASNGLUGLYASPGLNILEVALLEUILEASN
7   GLYARGASPILESERGLUHISTHRHISASP
8   GLNVALVALMETPHEILELYSALASERARG
9   GLUSERHISSERARGGLULEUALALEUVAL
10   ILEARGARGARG

Samples:

18.PTPN3_PDZ_HPV16_13C15N_300uM_130515: PDZ_PTPN3, [U-100% 13C; U-100% 15N], 0.3 mM; tris 50 mM; NaCl 150 mM; TCEP 0.5 mM

CondSet1: ionic strength: 0.200 M; pH: 7.500; pressure: 1.000 atm; temperature: 288.150 K

Experiments:

NameSampleSample stateSample conditions
3D HNCO18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1
2D 1H-15N HSQC/HMQC18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1
3D HNCA18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1
3D HNCACB18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1
3D HN(CO)CA18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1
Expt_44 (H[N[co[{CA|ca[C]}]]])18.PTPN3_PDZ_HPV16_13C15N_300uM_130515isotropicCondSet1

Software:

CcpNmr_Analysis v2.3, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UniProt P26045
AlphaFold E9PGU7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks