BMRB Entry 27643

Name: Chemical shifts of the WH domain of human ORC2
Published: 2018-11-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27643

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of the WH domain of human ORC2

Deposition date:

2018-10-09

Original release date:

2018-11-08

Authors:

Zhang, Jiahai

Citation:

Citation: Zhang, Jiahai; Guo, Qiong; Gao, Jun; Xu, Chao; Zhang, Beibei; Li, Mingwei; She, Jiaqi; Shi, Yunyu; Zhang, Zhiyong; Ruan, Ke; Wu, Jihui. "Structural insight into the unique dsDNA binding topology of the human ORC2 wing helix domain" FEBS J. 286, 2726-2736 (2019).
PubMed: 30963726

Assembly members:

Assembly members:
WH_Domain_of_Human_ORC2, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: peT28-MHL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WH_Domain_of_Human_ORC2: SLPLSSLTHVLRSLTPNARG IFRLLIKYQLDNQDNPSYIG LSFQDFYQQCREAFLVNSDL TLRAQLTEFRDHKLIRTKKG TDGVEYLLIPVDNGTLTDFL EKEEEEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
15N chemical shifts	114
1H chemical shifts	715

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WH DOmain of ORC2	1

Entities:

Entity 1, WH DOmain of ORC2 107 residues - Formula weight is not available

1

SER

LEU

PRO

LEU

SER

LEU

THR

HIS

VAL

2

LEU

ARG

SER

LEU

THR

PRO

ASN

ALA

ARG

GLY

3

ILE

PHE

ARG

LEU

ILE

LYS

TYR

GLN

LEU

4

ASP

ASN

GLN

ASP

ASN

PRO

SER

TYR

ILE

GLY

5

LEU

SER

PHE

GLN

ASP

PHE

TYR

GLN

CYS

6

ARG

GLU

ALA

PHE

LEU

VAL

ASN

SER

ASP

LEU

7

THR

LEU

ARG

ALA

GLN

LEU

THR

GLU

PHE

ARG

8

ASP

HIS

LYS

LEU

ILE

ARG

THR

LYS

GLY

9

THR

ASP

GLY

VAL

GLU

TYR

LEU

ILE

PRO

10

VAL

ASP

ASN

GLY

THR

LEU

THR

ASP

PHE

LEU

11

GLU

LYS

GLU

ALA

Samples:

sample_1: WH_Domain_of_Human_ORC2, [U-99% 13C; U-99% 15N], 0.8 mM; phosphate sodium 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks