BMRB Entry 27639
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27639
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Citation: Tang, Tzu-Chun; Kienlen-Campard, Pascal; Hu, Yi; Perrin, Florian; Opsomer, Remi; Octave, Jean-Noel; Constantinescu, Stefan; Smith, Steven. "Influence of the familial Alzheimer's disease-associated T43I mutation on the transmembrane structure and gamma-secretase processing of the C99 peptide" J. Biol. Chem. 294, 5854-5866 (2019).
PubMed: 30755484
Assembly members:
C55, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
C55: DAEFRHDSGYEVHHQKLVFF
AEDVGSNKGAIIGLMVGGVV
IATVIVITLVMLKKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 21 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Single polypeptide chain C55 | 1 |
Entities:
Entity 1, Single polypeptide chain C55 55 residues - Formula weight is not available
| 1 | ASP | ALA | GLU | PHE | ARG | HIS | ASP | SER | GLY | TYR | ||||
| 2 | GLU | VAL | HIS | HIS | GLN | LYS | LEU | VAL | PHE | PHE | ||||
| 3 | ALA | GLU | ASP | VAL | GLY | SER | ASN | LYS | GLY | ALA | ||||
| 4 | ILE | ILE | GLY | LEU | MET | VAL | GLY | GLY | VAL | VAL | ||||
| 5 | ILE | ALA | THR | VAL | ILE | VAL | ILE | THR | LEU | VAL | ||||
| 6 | MET | LEU | LYS | LYS | LYS |
Samples:
sample_1: C55, [U-13C], 50 mM
sample_conditions_1: ionic strength: 0.01 M; pH: 7.2; pressure: 1 atm; temperature: 210 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz