BMRB Entry 27638

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27638

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Title:
Backbone NMR assignment of the oleate bound form of Jug r 3, the non-specific lipid transfer protein from walnut.
Deposition date:
2018-10-05
Original release date:
2019-02-22
Authors:
Dubiela, Pawel; del Conte, Rebecca; Cantini, Francesca; Borowski, Tomasz; Aina, Roberta; Radauer, Christian; Bublin, Merima; Hoffmann-Sommergruber, Karin; Alessandri, Stefano
Citation:

Citation: Dubiela, Pawel; del Conte, Rebecca; Cantini, Francesca; Borowski, Tomasz; Aina, Roberta; Radauer, Christian; Bublin, Merima; Hoffmann-Sommergruber, Karin; Alessandri, Stefano. "Impact of lipid binding on the tertiary structure and allergenic potential of Jug r 3, the non-specific lipid transfer protein from walnut"  Sci. Rep. 9, .-2007 (2019).
PubMed: 30765752

Assembly members:

Assembly members:
Jug_r_3, polymer, 97 residues, Formula weight is not available
OLEIC ACID, non-polymer, 282.461 Da.

Natural source:

Natural source:   Common Name: English walnut   Taxonomy ID: 51240   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Juglans regia

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZaA-Jug r 3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Jug_r_3: EAEFVITCGQVASSVGSCIG YLRGTVPTVPPSCCNGVKSL NKAAATTADRQAACECLKKT SGSIPGLNPGLAAGLPGKCG VSVPYKISTSTNCKAVK

Data sets:
Data typeCount
13C chemical shifts128
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1jug r 31
2oleic acid2

Entities:

Entity 1, jug r 3 97 residues - Formula weight is not available

EAEF stretch N-terminal residues derived from the signal sequence cleavage site.

1   GLUALAGLUPHEVALILETHRCYSGLYGLN
2   VALALASERSERVALGLYSERCYSILEGLY
3   TYRLEUARGGLYTHRVALPROTHRVALPRO
4   PROSERCYSCYSASNGLYVALLYSSERLEU
5   ASNLYSALAALAALATHRTHRALAASPARG
6   GLNALAALACYSGLUCYSLEULYSLYSTHR
7   SERGLYSERILEPROGLYLEUASNPROGLY
8   LEUALAALAGLYLEUPROGLYLYSCYSGLY
9   VALSERVALPROTYRLYSILESERTHRSER
10   THRASNCYSLYSALAVALLYS

Entity 2, oleic acid - C18 H34 O2 - 282.461 Da.

1   OLA

Related Database Links:

UNIPROT C5H617 (C5H617_9ROSI)
AlphaFold C5H617

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks