BMRB Entry 27633

Name: Chemical shift assignments of the C-terminal domain of chicken H1.11L
Published: 2019-02-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27633

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments of the C-terminal domain of chicken H1.11L

Deposition date:

2018-09-27

Original release date:

2019-02-06

Authors:

Turner, Abigail; Watson, Matthew; Wilkins, Oscar; Cato, Laura; Travers, Andrew; Thomas, Jean; Stott, Katherine

Citation:

Citation: Turner, Abigail; Watson, Matthew; Wilkins, Oscar; Cato, Laura; Travers, Andrew; Thomas, Jean; Stott, Katherine. "Highly disordered histone H1-DNA model complexes and their condensates" Proc. Natl. Acad. Sci. U.S.A. 115, 11964-11969 (2018).
PubMed: 30301810

Assembly members:

Assembly members:
H1, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET13a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H1: KPGEVKEKAPKKKASAAKPK KPAAKKPAAAAKKPKKAVAV KKSPKKAKKPAASATKKSAK SPKKVTKAVKPKKAVAAKSP AKAKAVKPKAAKPKAAKPKA AKAKKAAAKKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	71
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	H1 C-terminal domain	1

Entities:

Entity 1, H1 C-terminal domain 111 residues - Formula weight is not available

1

LYS

PRO

GLY

GLU

VAL

LYS

GLU

LYS

ALA

PRO

2

LYS

ALA

SER

ALA

LYS

PRO

LYS

3

LYS

PRO

ALA

LYS

PRO

ALA

4

ALA

LYS

PRO

LYS

ALA

VAL

ALA

VAL

5

LYS

SER

PRO

LYS

ALA

LYS

PRO

6

ALA

SER

ALA

THR

LYS

SER

ALA

LYS

7

SER

PRO

LYS

VAL

THR

LYS

ALA

VAL

LYS

8

PRO

LYS

ALA

VAL

ALA

LYS

SER

PRO

9

ALA

LYS

ALA

LYS

ALA

VAL

LYS

PRO

LYS

ALA

10

ALA

LYS

PRO

LYS

ALA

LYS

PRO

LYS

ALA

11

ALA

LYS

ALA

LYS

ALA

LYS

12

LYS

Samples:

sample_1: H1, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 0.5 mM; NaCl 0.5 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

AZARA, Boucher - processing

Analysis vv2.4, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks