BMRB Entry 27630

Name: 1HN,15N,13CO,13CA and 13CB Chemical Shift Assignments for the FBP21(326-276) Fragment in Complex with the Brr2-CSec63 domain
Published: 2018-10-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27630

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3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1HN,15N,13CO,13CA and 13CB Chemical Shift Assignments for the FBP21(326-276) Fragment in Complex with the Brr2-CSec63 domain

Deposition date:

2018-09-26

Original release date:

2018-10-09

Authors:

Sticht, Jana; Freund, Christian

Citation:

Citation: Sticht, Jana; Bertazzon, Miriam; Henning, Lisa; Licha, Jan; Abualrous, Esam; Freund, Christian. "FBP21's C-Terminal Domain Remains Dynamic When Wrapped around the c-Sec63 Unit of Brr2 Helicase" Biophys. J. 116, 406-418 (2019).
PubMed: 30558886

Assembly members:

Assembly members:
FBP21(326-376), polymer, 54 residues, Formula weight is not available
Brr2-CSec63, polymer, 305 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FBP21(326-376): GAMEADGGGEPKVVFKEKTV TSLGVMADGVAPVFKKRRTE NGKSRNLRQRGDDQ
Brr2-CSec63: GPLGSPEFTKVRGLIEIISN AAEYENIPIRHHEDNLLRQL AQKVPHKLNNPKFNDPHVKT NLLLQAHLSRMQLSAELQSD TEEILSKAIRLIQACVDVLS SNGWLSPALAAMELAQMVTQ AMWSKDSYLKQLPHFTSEHI KRCTDKGVESVFDIMEMEDE ERNALLQLTDSQIADVARFC NRYPNIELSYEVVDKDSIRS GGPVVVLVQLEREEEVTGPV IAPLFPQKREEGWWVVIGDA KSNSLISIKRLTLQQKAKVK LDFVAPATGAHNYTLYFMSD AYMGCDQEYKFSVDVKEAET DSDSD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	133
15N chemical shifts	49
1H chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FBP21(326-376)	1
2	Brr2-CSec63	2

Entities:

Entity 1, FBP21(326-376) 54 residues - Formula weight is not available

Residues 1-3 (323-325) (GAM) derive from cloning, residues 4-54 (326-376) represent the last C-terminal residues of FBP21.

1

GLY

ALA

MET

GLU

ALA

ASP

GLY

GLU

2

PRO

LYS

VAL

PHE

LYS

GLU

LYS

THR

VAL

3

THR

SER

LEU

GLY

VAL

MET

ALA

ASP

GLY

VAL

4

ALA

PRO

VAL

PHE

LYS

ARG

THR

GLU

5

ASN

GLY

LYS

SER

ARG

ASN

LEU

ARG

GLN

ARG

6

GLY

ASP

GLN

Entity 2, Brr2-CSec63 305 residues - Formula weight is not available

Brr2-CSec63 covers residues 1840-2136 of human Brr2 helicase. The first 8 residues derive from cloning, Brr2-C-Sec sequece starts with residue 9T, which corresponds to 1840T.

1

GLY

PRO

LEU

GLY

SER

PRO

GLU

PHE

THR

LYS

2

VAL

ARG

GLY

LEU

ILE

GLU

ILE

SER

ASN

3

ALA

GLU

TYR

GLU

ASN

ILE

PRO

ILE

ARG

4

HIS

GLU

ASP

ASN

LEU

ARG

GLN

LEU

5

ALA

GLN

LYS

VAL

PRO

HIS

LYS

LEU

ASN

6

PRO

LYS

PHE

ASN

ASP

PRO

HIS

VAL

LYS

THR

7

ASN

LEU

GLN

ALA

HIS

LEU

SER

ARG

8

MET

GLN

LEU

SER

ALA

GLU

LEU

GLN

SER

ASP

9

THR

GLU

ILE

LEU

SER

LYS

ALA

ILE

ARG

10

LEU

ILE

GLN

ALA

CYS

VAL

ASP

VAL

LEU

SER

11

SER

ASN

GLY

TRP

LEU

SER

PRO

ALA

LEU

ALA

12

ALA

MET

GLU

LEU

ALA

GLN

MET

VAL

THR

GLN

13

ALA

MET

TRP

SER

LYS

ASP

SER

TYR

LEU

LYS

14

GLN

LEU

PRO

HIS

PHE

THR

SER

GLU

HIS

ILE

15

LYS

ARG

CYS

THR

ASP

LYS

GLY

VAL

GLU

SER

16

VAL

PHE

ASP

ILE

MET

GLU

MET

GLU

ASP

GLU

17

GLU

ARG

ASN

ALA

LEU

GLN

LEU

THR

ASP

18

SER

GLN

ILE

ALA

ASP

VAL

ALA

ARG

PHE

CYS

19

ASN

ARG

TYR

PRO

ASN

ILE

GLU

LEU

SER

TYR

20

GLU

VAL

ASP

LYS

ASP

SER

ILE

ARG

SER

21

GLY

PRO

VAL

LEU

VAL

GLN

LEU

22

GLU

ARG

GLU

VAL

THR

GLY

PRO

VAL

23

ILE

ALA

PRO

LEU

PHE

PRO

GLN

LYS

ARG

GLU

24

GLU

GLY

TRP

VAL

ILE

GLY

ASP

ALA

25

LYS

SER

ASN

SER

LEU

ILE

SER

ILE

LYS

ARG

26

LEU

THR

LEU

GLN

LYS

ALA

LYS

VAL

LYS

27

LEU

ASP

PHE

VAL

ALA

PRO

ALA

THR

GLY

ALA

28

HIS

ASN

TYR

THR

LEU

TYR

PHE

MET

SER

ASP

29

ALA

TYR

MET

GLY

CYS

ASP

GLN

GLU

TYR

LYS

30

PHE

SER

VAL

ASP

VAL

LYS

GLU

ALA

GLU

THR

31

ASP

SER

ASP

SER

ASP

Samples:

sample_1: FBP21(326-376), [U-13C; U-15N; U-2H], 140 uM; sodium phosphate 20 mM; sodium chloride 100 mM; Brr2-CSec63 420 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - processing

CcpNMR_Analysis v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks