BMRB Entry 27629

Name: 1HN,15N,13CO,13CA and 13CB Chemical Shift Assignments for the FBP21(326-276) Fragment
Published: 2018-10-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27629

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1HN,15N,13CO,13CA and 13CB Chemical Shift Assignments for the FBP21(326-276) Fragment

Deposition date:

2018-09-26

Original release date:

2018-10-09

Authors:

Sticht, Jana; Freund, Christian

Citation:

Citation: Sticht, Jana; Bertazzon, Miriam; Henning, Lisa; Licha, Jan; Abualrous, Esam; Freund, Christian. "FBP21's C-Terminal Domain Remains Dynamic When Wrapped around the c-Sec63 Unit of Brr2 Helicase" Biophys. J. 116, 406-418 (2019).
PubMed: 30558886

Assembly members:

Assembly members:
FBP21(326-376), polymer, 54 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FBP21(326-376): GAMEADGGGEPKVVFKEKTV TSLGVMADGVAPVFKKRRTE NGKSRNLRQRGDDQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	138
15N chemical shifts	47
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FBP21(326-376)	1

Entities:

Entity 1, FBP21(326-376) 54 residues - Formula weight is not available

Residues 1-3 (GAM) derive from cloning, residues 4-54 represent the last C-terminal residues of FBP21.

1

GLY

ALA

MET

GLU

ALA

ASP

GLY

GLU

2

PRO

LYS

VAL

PHE

LYS

GLU

LYS

THR

VAL

3

THR

SER

LEU

GLY

VAL

MET

ALA

ASP

GLY

VAL

4

ALA

PRO

VAL

PHE

LYS

ARG

THR

GLU

5

ASN

GLY

LYS

SER

ARG

ASN

LEU

ARG

GLN

ARG

6

GLY

ASP

GLN

Samples:

sample_1: FBP21(326-376), [U-13C; U-15N; U-2H], 140 uM; sodium phosphate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - processing

CcpNMR_Analysis v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks