BMRB Entry 27627

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27627

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Title:
Backbone Assignment Ubl45 domain of USP7
Deposition date:
2018-09-26
Original release date:
2019-01-14
Authors:
Kim, Robbert; van Ingen, Hugo; Sixma, Titia; Geurink, Paul; Mulder, Monique; Fish, Alexander; Ekkebus, Reggy; El Oualid, Farid; van Dijk, Willem; van Dalen, Duco; Ovaa, Huib
Citation:

Citation: Kim, Robbert; Geurink, Paul; Mulder, Monique; Fish, Alexander; Ekkebus, Reggy; El Oualid, Farid; van Dijk, Willem; van Dalen, Duco; Ovaa, Huib; van Ingen, Hugo; Sixma, Titia. "Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity."  Nat Commun 10, 231-231 (2019).
PubMed: 30651545

Assembly members:

Assembly members:
Ubl45, polymer, 226 residues, 26584.4325 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubl45: GPLGSPEFPGRLENRRSFKC IWLNSQFREEEITLYPDKHG CVRDLLEECKKAVELGEKAS GKLRLLEIVSYKIIGVHQED ELLECLSPATSRTFRIEEIP LDQVDIDKENEMLVTVAHFH KEVFGTFGIPFLLRIHQGEH FREVMKRIQSLLDIQEKEFE KFKFAIVMTGRHQYINEDEY EVNLKDFEPQPGNMSHPRPW LGLDHFNKAPKRSRYTYLEK AIKIHN

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts185
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubl451

Entities:

Entity 1, Ubl45 226 residues - 26584.4325 Da.

First 12 residues represent a non-native affinity tag

1   GLYPROLEUGLYSERPROGLUPHEPROGLY
2   ARGLEUGLUASNARGARGSERPHELYSCYS
3   ILETRPLEUASNSERGLNPHEARGGLUGLU
4   GLUILETHRLEUTYRPROASPLYSHISGLY
5   CYSVALARGASPLEULEUGLUGLUCYSLYS
6   LYSALAVALGLULEUGLYGLULYSALASER
7   GLYLYSLEUARGLEULEUGLUILEVALSER
8   TYRLYSILEILEGLYVALHISGLNGLUASP
9   GLULEULEUGLUCYSLEUSERPROALATHR
10   SERARGTHRPHEARGILEGLUGLUILEPRO
11   LEUASPGLNVALASPILEASPLYSGLUASN
12   GLUMETLEUVALTHRVALALAHISPHEHIS
13   LYSGLUVALPHEGLYTHRPHEGLYILEPRO
14   PHELEULEUARGILEHISGLNGLYGLUHIS
15   PHEARGGLUVALMETLYSARGILEGLNSER
16   LEULEUASPILEGLNGLULYSGLUPHEGLU
17   LYSPHELYSPHEALAILEVALMETTHRGLY
18   ARGHISGLNTYRILEASNGLUASPGLUTYR
19   GLUVALASNLEULYSASPPHEGLUPROGLN
20   PROGLYASNMETSERHISPROARGPROTRP
21   LEUGLYLEUASPHISPHEASNLYSALAPRO
22   LYSARGSERARGTYRTHRTYRLEUGLULYS
23   ALAILELYSILEHISASN

Samples:

sample_1: Ubl45, [U-13C; U-15N; U-2H], 180 uM; HEPES 50 mM; sodium chloride 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
HNcoCB_2 (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, collection

Biospin v2.4, Bruker Biospin - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

. Q93009

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks