BMRB Entry 27627

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27627

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Title:
Backbone Assignment Ubl45 domain of USP7
Deposition date:
2018-09-26
Original release date:
2019-01-14
Authors:
Kim, Robbert; van Ingen, Hugo; Sixma, Titia; Geurink, Paul; Mulder, Monique; Fish, Alexander; Ekkebus, Reggy; El Oualid, Farid; van Dijk, Willem; van Dalen, Duco; Ovaa, Huib
Citation:

Citation: Kim, Robbert; Geurink, Paul; Mulder, Monique; Fish, Alexander; Ekkebus, Reggy; El Oualid, Farid; van Dijk, Willem; van Dalen, Duco; Ovaa, Huib; van Ingen, Hugo; Sixma, Titia. "Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity."  Nat Commun 10, 231-231 (2019).
PubMed: 30651545

Assembly members:

Assembly members:
Ubl45, polymer, 226 residues, 26584.4325 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubl45: GPLGSPEFPGRLENRRSFKC IWLNSQFREEEITLYPDKHG CVRDLLEECKKAVELGEKAS GKLRLLEIVSYKIIGVHQED ELLECLSPATSRTFRIEEIP LDQVDIDKENEMLVTVAHFH KEVFGTFGIPFLLRIHQGEH FREVMKRIQSLLDIQEKEFE KFKFAIVMTGRHQYINEDEY EVNLKDFEPQPGNMSHPRPW LGLDHFNKAPKRSRYTYLEK AIKIHN

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts185
1H chemical shifts185

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubl451

Entities:

Entity 1, Ubl45 226 residues - 26584.4325 Da.

First 12 residues represent a non-native affinity tag

1   GLYPROLEUGLYSERPROGLUPHEPROGLY
2   ARGLEUGLUASNARGARGSERPHELYSCYS
3   ILETRPLEUASNSERGLNPHEARGGLUGLU
4   GLUILETHRLEUTYRPROASPLYSHISGLY
5   CYSVALARGASPLEULEUGLUGLUCYSLYS
6   LYSALAVALGLULEUGLYGLULYSALASER
7   GLYLYSLEUARGLEULEUGLUILEVALSER
8   TYRLYSILEILEGLYVALHISGLNGLUASP
9   GLULEULEUGLUCYSLEUSERPROALATHR
10   SERARGTHRPHEARGILEGLUGLUILEPRO
11   LEUASPGLNVALASPILEASPLYSGLUASN
12   GLUMETLEUVALTHRVALALAHISPHEHIS
13   LYSGLUVALPHEGLYTHRPHEGLYILEPRO
14   PHELEULEUARGILEHISGLNGLYGLUHIS
15   PHEARGGLUVALMETLYSARGILEGLNSER
16   LEULEUASPILEGLNGLULYSGLUPHEGLU
17   LYSPHELYSPHEALAILEVALMETTHRGLY
18   ARGHISGLNTYRILEASNGLUASPGLUTYR
19   GLUVALASNLEULYSASPPHEGLUPROGLN
20   PROGLYASNMETSERHISPROARGPROTRP
21   LEUGLYLEUASPHISPHEASNLYSALAPRO
22   LYSARGSERARGTYRTHRTYRLEUGLULYS
23   ALAILELYSILEHISASN

Related Database Links:

. Q93009

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks