BMRB Entry 27625

Name: 1H, 13C, 15N assignment of the CD44 cytoplasmatic tail (669-742)
Published: 2019-01-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27625

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N assignment of the CD44 cytoplasmatic tail (669-742)

Deposition date:

2018-09-25

Original release date:

2019-01-09

Authors:

Fruehbauer, Benjamin; Mateos, Borja; Konrat, Robert

Citation:

Citation: Fruehbauer, Benjamin; Mateos, Borja; Konrat, Robert. "1H, 15N, 13C resonance assignment of the human CD44 cytoplasmic tail (669-742)" Biomol. NMR Assignments 13, 109-113 (2019).
PubMed: 30474821

Assembly members:

Assembly members:
CD44ctail, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD44ctail: GAMGAVNSRRRCGQKKKLVI NSGNGAVEDRKPSGLNGEAS KSQEMVHLVNKESSETPDQF MTADETRNLQNVDMKIGV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	218
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD44ctail	1

Entities:

Entity 1, CD44ctail 78 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ALA

VAL

ASN

SER

ARG

2

ARG

CYS

GLY

GLN

LYS

LEU

VAL

ILE

3

ASN

SER

GLY

ASN

GLY

ALA

VAL

GLU

ASP

ARG

4

LYS

PRO

SER

GLY

LEU

ASN

GLY

GLU

ALA

SER

5

LYS

SER

GLN

GLU

MET

VAL

HIS

LEU

VAL

ASN

6

LYS

GLU

SER

GLU

THR

PRO

ASP

GLN

PHE

7

MET

THR

ALA

ASP

GLU

THR

ARG

ASN

LEU

GLN

8

ASN

VAL

ASP

MET

LYS

ILE

GLY

VAL

Samples:

sample_1: CD44ctail, [U-100% 13C; U-100% 15N], 4.5 mM; D2O, [U-2H], 10%; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, Bruker Biospin, CCPN - chemical shift assignment, collection, processing

NMR spectrometers:

Bruker Neo 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks