BMRB Entry 27624

Name: Klebsiella pneumoniae sigma4 of sigmaS fused to the beta-flap-tip helix
Published: 2019-08-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27624

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Klebsiella pneumoniae sigma4 of sigmaS fused to the beta-flap-tip helix

Deposition date:

2018-09-24

Original release date:

2019-08-15

Authors:

Lou, Yuan-Chao; Chen, Chinpan

Citation:

Citation: Lou, Yuan-Chao; Chou, Chun-Chi; Yeh, Hsin-Hong; Chien, Chia-Yu; Sadotra, Sushant; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis for -35 element recognition by sigma4 chimera proteins and their interactions with PmrA response regulator" Proteins 88, 69-81 (2020).
PubMed: 31293000

Assembly members:

Assembly members:
sigma4-Sc_monomer, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Klebsiella pneumoniae Taxonomy ID: 573 Superkingdom: Bacteria Kingdom: not available Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-GB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sigma4-Sc_monomer: GAMGGPEDTTQDDDMKQSIV KWLFELNAKQREVLARRFGL LGYEAATLEDVGREIGLTRE RVRQIQVEGLRRLREILQGQ GLNIEALFREGSGSKGETQL TPEEKLLRAIFGEKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	123
1H chemical shifts	789

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sigma4-Sc monomer	1

Entities:

Entity 1, sigma4-Sc monomer 115 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

PRO

GLU

ASP

THR

2

GLN

ASP

MET

LYS

GLN

SER

ILE

VAL

3

LYS

TRP

LEU

PHE

GLU

LEU

ASN

ALA

LYS

GLN

4

ARG

GLU

VAL

LEU

ALA

ARG

PHE

GLY

LEU

5

LEU

GLY

TYR

GLU

ALA

THR

LEU

GLU

ASP

6

VAL

GLY

ARG

GLU

ILE

GLY

LEU

THR

ARG

GLU

7

ARG

VAL

ARG

GLN

ILE

GLN

VAL

GLU

GLY

LEU

8

ARG

LEU

ARG

GLU

ILE

LEU

GLN

GLY

GLN

9

GLY

LEU

ASN

ILE

GLU

ALA

LEU

PHE

ARG

GLU

10

GLY

SER

GLY

SER

LYS

GLY

GLU

THR

GLN

LEU

11

THR

PRO

GLU

LYS

LEU

ARG

ALA

ILE

12

PHE

GLY

GLU

LYS

ALA

Samples:

sample_1: sigma4-Sc monomer, [U-99% 13C; U-99% 15N], 1.5 mM; Na2HPO4 20 mM; NaCl 30 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks