BMRB Entry 27623

Name: Klebsiella pneumoniae sigma4 of sigma70 fused to the beta-flap-tip helix
Published: 2019-08-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27623

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Klebsiella pneumoniae sigma4 of sigma70 fused to the beta-flap-tip helix

Deposition date:

2018-09-24

Original release date:

2019-08-15

Authors:

Lou, Yuan-Chao; Chen, Chinpan

Citation:

Citation: Lou, Yuan-Chao; Chou, Chun-Chi; Yeh, Hsin-Hong; Chien, Chia-Yu; Sadotra, Sushant; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis for -35 element recognition by sigma4 chimera proteins and their interactions with PmrA response regulator" Proteins 88, 69-81 (2020).
PubMed: 31293000

Assembly members:

Assembly members:
sigma4-70c, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Klebsiella pneumoniae Taxonomy ID: 573 Superkingdom: Bacteria Kingdom: not available Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-GB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sigma4-70c: GLPLDSATTESLRAATHDVL AGLTAREAKVLRMRFGIDMN TDHTLEEVGKQFDVTRERIR QIEAKALRKLRHPSRSEVLR SFLGSGWKGETQLTPEEKLL RAIFGEKA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	377
15N chemical shifts	92
1H chemical shifts	476

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sigma4-70c monomer	1

Entities:

Entity 1, sigma4-70c monomer 108 residues - Formula weight is not available

1

GLY

LEU

PRO

LEU

ASP

SER

ALA

THR

GLU

2

SER

LEU

ARG

ALA

THR

HIS

ASP

VAL

LEU

3

ALA

GLY

LEU

THR

ALA

ARG

GLU

ALA

LYS

VAL

4

LEU

ARG

MET

ARG

PHE

GLY

ILE

ASP

MET

ASN

5

THR

ASP

HIS

THR

LEU

GLU

VAL

GLY

LYS

6

GLN

PHE

ASP

VAL

THR

ARG

GLU

ARG

ILE

ARG

7

GLN

ILE

GLU

ALA

LYS

ALA

LEU

ARG

LYS

LEU

8

ARG

HIS

PRO

SER

ARG

SER

GLU

VAL

LEU

ARG

9

SER

PHE

LEU

GLY

SER

GLY

TRP

LYS

GLY

GLU

10

THR

GLN

LEU

THR

PRO

GLU

LYS

LEU

11

ARG

ALA

ILE

PHE

GLY

GLU

LYS

ALA

Samples:

sample_1: sigma4-70c, [U-99% 13C; U-99% 15N], 1.2 mM; Na2HPO4 20 mM; NaCl 30 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks