BMRB Entry 27619

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27619

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Title:
Backbone assignments of the bacterial tRNA-(N1G37) methyltransferase (TrmD)
Deposition date:
2018-09-19
Original release date:
2019-07-10
Authors:
Li, Yan; Ng, Hui Qi; Kang, CongBao
Citation:

Citation: Li, Yan; Zhong, Wenhe; Koay, Ann Zhufang; Ng, Hui Qi; Nah, Qianhui; Wong, Yee Hwa; Hill, Jeffrey; Lescar, Julien; Dedon, Peter; Kang, CongBao. "Backbone resonance assignment for the full length tRNA-(N"  Biomol. NMR Assign. 13, 327-332 (2019).
PubMed: 31175551

Assembly members:

Assembly members:
bacterial_tRNA-(N1G37)_methyltransferase_(TrmD), polymer, 258 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bacterial_tRNA-(N1G37)_methyltransferase_(TrmD): MDKRLWVGVVSIFPEMFRAI SDYGITSRAVKQGLLTLTCW NPRVYTEDRHQTVDDRPFGG GPGMVMKIKPLEGALADARQ AAGGRKAKVIYLSPQGRQLT QAGVRELAEEEALILIAGRY EGIDERFIEEHVDEEWSIGD YVLSGGELPAMVLVDAVTRL LPGALGHADSAEEDSFTDGL LDCPHYTRPEVYADKRVPEV LLSGNHEHIRRWRLQQALGR TWERRADLLDSRSLSGEEQK LLAEYIRQRDDSHHHHHH

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts223
1H chemical shifts223

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bacterial tRNA-(N1G37) methyltransferase (TrmD)1

Entities:

Entity 1, bacterial tRNA-(N1G37) methyltransferase (TrmD) 258 residues - Formula weight is not available

1   METASPLYSARGLEUTRPVALGLYVALVAL
2   SERILEPHEPROGLUMETPHEARGALAILE
3   SERASPTYRGLYILETHRSERARGALAVAL
4   LYSGLNGLYLEULEUTHRLEUTHRCYSTRP
5   ASNPROARGVALTYRTHRGLUASPARGHIS
6   GLNTHRVALASPASPARGPROPHEGLYGLY
7   GLYPROGLYMETVALMETLYSILELYSPRO
8   LEUGLUGLYALALEUALAASPALAARGGLN
9   ALAALAGLYGLYARGLYSALALYSVALILE
10   TYRLEUSERPROGLNGLYARGGLNLEUTHR
11   GLNALAGLYVALARGGLULEUALAGLUGLU
12   GLUALALEUILELEUILEALAGLYARGTYR
13   GLUGLYILEASPGLUARGPHEILEGLUGLU
14   HISVALASPGLUGLUTRPSERILEGLYASP
15   TYRVALLEUSERGLYGLYGLULEUPROALA
16   METVALLEUVALASPALAVALTHRARGLEU
17   LEUPROGLYALALEUGLYHISALAASPSER
18   ALAGLUGLUASPSERPHETHRASPGLYLEU
19   LEUASPCYSPROHISTYRTHRARGPROGLU
20   VALTYRALAASPLYSARGVALPROGLUVAL
21   LEULEUSERGLYASNHISGLUHISILEARG
22   ARGTRPARGLEUGLNGLNALALEUGLYARG
23   THRTRPGLUARGARGALAASPLEULEUASP
24   SERARGSERLEUSERGLYGLUGLUGLNLYS
25   LEULEUALAGLUTYRILEARGGLNARGASP
26   ASPSERHISHISHISHISHISHIS

Samples:

sample_1: bacterial RNA-(N1G37) methyltransferase (TrmD), [U-13C; U-15N; U-2H], 0.5 mM; sodium phosphate 20 mM; potassium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 170 mM; pH: 7.2; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks