BMRB Entry 27618

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Dss1
Published: 2018-10-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27618

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Dss1

Deposition date:

2018-09-19

Original release date:

2018-10-08

Authors:

Hendus-Altenburger, Ruth; Kragelund, Birthe; Rosner, Heike

Citation:

Citation: Schenstrom, Signe; Rebula, Caio; Tatham, Michael; Hendus-Altenburger, Ruth; Jourdain, Isabelle; Hay, Ronald; Kragelund, Birthe; Hartmann-Petersen, Rasmus. "Expanded Interactome of the Intrinsically Disordered Protein Dss1" Cell Rep. 25, 862-870 (2018).
PubMed: 30355493

Assembly members:

Assembly members:
Dss1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dss1: GPLGSMSRAALPSLENLEDD DEFEDFATENWPMKDTELDT GDDTLWENNWDDEDIGDDDF SVQLQAELKKKGVAAN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	204
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dss1	1

Entities:

Entity 1, Dss1 76 residues - Formula weight is not available

Residues 1-5 represent a non-native affinity tag

1

GLY

PRO

LEU

GLY

SER

MET

SER

ARG

ALA

2

LEU

PRO

SER

LEU

GLU

ASN

LEU

GLU

ASP

3

ASP

GLU

PHE

GLU

ASP

PHE

ALA

THR

GLU

ASN

4

TRP

PRO

MET

LYS

ASP

THR

GLU

LEU

ASP

THR

5

GLY

ASP

THR

LEU

TRP

GLU

ASN

TRP

6

ASP

GLU

ASP

ILE

GLY

ASP

PHE

7

SER

VAL

GLN

LEU

GLN

ALA

GLU

LEU

LYS

8

LYS

GLY

VAL

ALA

ASN

Samples:

sample_1: Dss1, [U-13C; U-15N], 340 uM; Tris/HCl 25 mM; sodium chloride 50 mM; D2O, [U-99% 2H], 10%; DSS 0.5 mM

sample_conditions_1: ionic strength: 71 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, CCPN - chemical shift assignment, processing

NMR spectrometers:

Varian INOVA 800 MHz
Bruker Avance 600 MHz