BMRB Entry 27611
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27611
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Citation: Panteleev, Pavel; Tsarev, Andrey; Bolosov, Ilia; Paramonov, Alexander; Marggraf, Mariana; Sychev, Sergey; Shenkarev, Zakhar; Ovchinnikova, Tatiana. "Novel Antimicrobial Peptides from the Arctic Polychaeta" Mar. Drugs 16, E401-E401 (2018).
PubMed: 30360541
Assembly members:
Nicomacin, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEM-T
Entity Sequences (FASTA):
Nicomacin: GFWSSVWDGAKNVGTAIIKN
AKVCVYAVCVSHK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 79 |
| 1H chemical shifts | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nicomacin | 1 |
Entities:
Entity 1, Nicomacin 33 residues - Formula weight is not available
| 1 | GLY | PHE | TRP | SER | SER | VAL | TRP | ASP | GLY | ALA | ||||
| 2 | LYS | ASN | VAL | GLY | THR | ALA | ILE | ILE | LYS | ASN | ||||
| 3 | ALA | LYS | VAL | CYS | VAL | TYR | ALA | VAL | CYS | VAL | ||||
| 4 | SER | HIS | LYS |
Samples:
sample_1: Nicomacin 0.4 mM
sample_conditions_1: ionic strength: 20 mM; pH: 4.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz