BMRB Entry 27607

Name: Sequence specific chemical shift assignments of the Caenorhabditis elegans SAS-6 N-terminal domain
Published: 2019-02-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27607

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Sequence specific chemical shift assignments of the Caenorhabditis elegans SAS-6 N-terminal domain

Deposition date:

2018-09-13

Original release date:

2019-02-15

Authors:

Erat, Michele; Vakonakis, Ioannis

Citation:

Citation: Busch, Julia; Erat, Michele; Blank, Iris; Musgaard, Maria; Biggin, Philip; Vakonakis, Ioannis. "A dynamically interacting flexible loop assists oligomerisation of the Caenorhabditis elegans centriolar protein SAS-6" Sci. Rep. 9, 3526-3526 (2019).
PubMed: 30837637

Assembly members:

Assembly members:
CeSAS-6N, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CeSAS-6N: GSMTSKIALFDQTLIASLLQ PLSLNQPDFKAYKTKVKLKI SEQRNETSGEKELKFEISRS DDFEFLFSETLNNEKYQILA RDHDLTVDFDAFPKVIIQHL LCKNIVKNLEEDGEVDARKK AGYHEIADPGKPTEINIILD AEKNFCSFELFSKTPESKGK IFSIKLHAVR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	320
15N chemical shifts	153
1H chemical shifts	153

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CeSAS-6N	1

Entities:

Entity 1, CeSAS-6N 170 residues - Formula weight is not available

Residues 1 and 2 are cloning artefacts. Entry includes two mutations, S123E and I154E, compared to database sequence.

1	GLY	SER	MET	THR	SER	LYS	ILE	ALA	LEU	PHE
2	ASP	GLN	THR	LEU	ILE	ALA	SER	LEU	LEU	GLN
3	PRO	LEU	SER	LEU	ASN	GLN	PRO	ASP	PHE	LYS
4	ALA	TYR	LYS	THR	LYS	VAL	LYS	LEU	LYS	ILE
5	SER	GLU	GLN	ARG	ASN	GLU	THR	SER	GLY	GLU
6	LYS	GLU	LEU	LYS	PHE	GLU	ILE	SER	ARG	SER
7	ASP	ASP	PHE	GLU	PHE	LEU	PHE	SER	GLU	THR
8	LEU	ASN	ASN	GLU	LYS	TYR	GLN	ILE	LEU	ALA
9	ARG	ASP	HIS	ASP	LEU	THR	VAL	ASP	PHE	ASP
10	ALA	PHE	PRO	LYS	VAL	ILE	ILE	GLN	HIS	LEU
11	LEU	CYS	LYS	ASN	ILE	VAL	LYS	ASN	LEU	GLU
12	GLU	ASP	GLY	GLU	VAL	ASP	ALA	ARG	LYS	LYS
13	ALA	GLY	TYR	HIS	GLU	ILE	ALA	ASP	PRO	GLY
14	LYS	PRO	THR	GLU	ILE	ASN	ILE	ILE	LEU	ASP
15	ALA	GLU	LYS	ASN	PHE	CYS	SER	PHE	GLU	LEU
16	PHE	SER	LYS	THR	PRO	GLU	SER	LYS	GLY	LYS
17	ILE	PHE	SER	ILE	LYS	LEU	HIS	ALA	VAL	ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

UNP	O62479
AlphaFold	O62479

BMRB Entry 27607

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	MET	THR	SER	LYS	ILE	ALA	LEU	PHE
2	ASP	GLN	THR	LEU	ILE	ALA	SER	LEU	LEU	GLN
3	PRO	LEU	SER	LEU	ASN	GLN	PRO	ASP	PHE	LYS
4	ALA	TYR	LYS	THR	LYS	VAL	LYS	LEU	LYS	ILE
5	SER	GLU	GLN	ARG	ASN	GLU	THR	SER	GLY	GLU
6	LYS	GLU	LEU	LYS	PHE	GLU	ILE	SER	ARG	SER
7	ASP	ASP	PHE	GLU	PHE	LEU	PHE	SER	GLU	THR
8	LEU	ASN	ASN	GLU	LYS	TYR	GLN	ILE	LEU	ALA
9	ARG	ASP	HIS	ASP	LEU	THR	VAL	ASP	PHE	ASP
10	ALA	PHE	PRO	LYS	VAL	ILE	ILE	GLN	HIS	LEU
11	LEU	CYS	LYS	ASN	ILE	VAL	LYS	ASN	LEU	GLU
12	GLU	ASP	GLY	GLU	VAL	ASP	ALA	ARG	LYS	LYS
13	ALA	GLY	TYR	HIS	GLU	ILE	ALA	ASP	PRO	GLY
14	LYS	PRO	THR	GLU	ILE	ASN	ILE	ILE	LEU	ASP
15	ALA	GLU	LYS	ASN	PHE	CYS	SER	PHE	GLU	LEU
16	PHE	SER	LYS	THR	PRO	GLU	SER	LYS	GLY	LYS
17	ILE	PHE	SER	ILE	LYS	LEU	HIS	ALA	VAL	ARG

1	GLY	SER	MET	THR	SER	LYS	ILE	ALA	LEU	PHE
2	ASP	GLN	THR	LEU	ILE	ALA	SER	LEU	LEU	GLN
3	PRO	LEU	SER	LEU	ASN	GLN	PRO	ASP	PHE	LYS
4	ALA	TYR	LYS	THR	LYS	VAL	LYS	LEU	LYS	ILE
5	SER	GLU	GLN	ARG	ASN	GLU	THR	SER	GLY	GLU
6	LYS	GLU	LEU	LYS	PHE	GLU	ILE	SER	ARG	SER
7	ASP	ASP	PHE	GLU	PHE	LEU	PHE	SER	GLU	THR
8	LEU	ASN	ASN	GLU	LYS	TYR	GLN	ILE	LEU	ALA
9	ARG	ASP	HIS	ASP	LEU	THR	VAL	ASP	PHE	ASP
10	ALA	PHE	PRO	LYS	VAL	ILE	ILE	GLN	HIS	LEU
11	LEU	CYS	LYS	ASN	ILE	VAL	LYS	ASN	LEU	GLU
12	GLU	ASP	GLY	GLU	VAL	ASP	ALA	ARG	LYS	LYS
13	ALA	GLY	TYR	HIS	GLU	ILE	ALA	ASP	PRO	GLY
14	LYS	PRO	THR	GLU	ILE	ASN	ILE	ILE	LEU	ASP
15	ALA	GLU	LYS	ASN	PHE	CYS	SER	PHE	GLU	LEU
16	PHE	SER	LYS	THR	PRO	GLU	SER	LYS	GLY	LYS
17	ILE	PHE	SER	ILE	LYS	LEU	HIS	ALA	VAL	ARG

1	GLY	SER	MET	THR	SER	LYS	ILE	ALA	LEU	PHE
2	ASP	GLN	THR	LEU	ILE	ALA	SER	LEU	LEU	GLN
3	PRO	LEU	SER	LEU	ASN	GLN	PRO	ASP	PHE	LYS
4	ALA	TYR	LYS	THR	LYS	VAL	LYS	LEU	LYS	ILE
5	SER	GLU	GLN	ARG	ASN	GLU	THR	SER	GLY	GLU
6	LYS	GLU	LEU	LYS	PHE	GLU	ILE	SER	ARG	SER
7	ASP	ASP	PHE	GLU	PHE	LEU	PHE	SER	GLU	THR
8	LEU	ASN	ASN	GLU	LYS	TYR	GLN	ILE	LEU	ALA
9	ARG	ASP	HIS	ASP	LEU	THR	VAL	ASP	PHE	ASP
10	ALA	PHE	PRO	LYS	VAL	ILE	ILE	GLN	HIS	LEU
11	LEU	CYS	LYS	ASN	ILE	VAL	LYS	ASN	LEU	GLU
12	GLU	ASP	GLY	GLU	VAL	ASP	ALA	ARG	LYS	LYS
13	ALA	GLY	TYR	HIS	GLU	ILE	ALA	ASP	PRO	GLY
14	LYS	PRO	THR	GLU	ILE	ASN	ILE	ILE	LEU	ASP
15	ALA	GLU	LYS	ASN	PHE	CYS	SER	PHE	GLU	LEU
16	PHE	SER	LYS	THR	PRO	GLU	SER	LYS	GLY	LYS
17	ILE	PHE	SER	ILE	LYS	LEU	HIS	ALA	VAL	ARG