BMRB Entry 27606

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27606

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Title:
Backbone and side chain resonance assignment of the NZF domain of HOIL-1L
Deposition date:
2018-09-13
Original release date:
2018-09-20
Authors:
Ishii, Naoki; Walinda, Erik; Iwakawa, Naoto; Morimoto, Daichi; Sugase, Kenji; Shirakawa, Masahiro
Citation:

Citation: Ishii, Naoki; Walinda, Erik; Iwakawa, Naoto; Morimoto, Daichi; Iwai, Kazuhiro; Sugase, Kenji; Shirakawa, Masahiro. "NMR resonance assignments of the NZF domain of mouse HOIL-1L free and bound to linear di-ubiquitin."  Biomol. NMR Assign. 13, 149-153 (2019).
PubMed: 30569274

Assembly members:

Assembly members:
NZF_domain, polymer, 66 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NZF_domain: GPLGSPEPVGWQCPGCTFIN KPTRPGCEMCCRARPETYQI PASYQPDEEERARLAGEEEA LRQYQQ

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts63
1H chemical shifts429

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HOIL-1L1
2Zinc2

Entities:

Entity 1, HOIL-1L 66 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROGLUPROVALGLY
2   TRPGLNCYSPROGLYCYSTHRPHEILEASN
3   LYSPROTHRARGPROGLYCYSGLUMETCYS
4   CYSARGALAARGPROGLUTHRTYRGLNILE
5   PROALASERTYRGLNPROASPGLUGLUGLU
6   ARGALAARGLEUALAGLYGLUGLUGLUALA
7   LEUARGGLNTYRGLNGLN

Entity 2, Zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: NZF domain, [U-100% 13C; U-100% 15N], 1.0 mM; HEPES 20 mM; sodium chloride 50 mM; TCEP 1.0 mM; D2O 5.0%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D 15N NOESY-HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphatic aliasedsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D H(CC)(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 13C NOESY-HSQC aliphaticsample_1isotropicsample_conditions_1
3D 13C NOESY-HSQC aromaticsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks