BMRB Entry 27603

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27603

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Title:
Partial backbone assignments of Cse4 protein in 5M urea
Deposition date:
2018-09-11
Original release date:
2019-01-09
Authors:
Kumar, Ashutosh; Malik, Nikita
Citation:

Citation: Malik, Nikita; Dantu, Sarath Chandra; Shukla, Shivangi; Kombrabail, Mamta; Ghosh, Santanu Kumar; Krishnamoorthy, Guruswamy; Kumar, Ashutosh. "Conformational flexibility of histone variant CENP-A"  J. Biol. Chem. 293, 20273-20284 (2018).
PubMed: 30381395

Assembly members:

Assembly members:
Cse4, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKS387

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cse4: MSSKQQWVSSAIQSDSSGRS LSNVNRLAGDQQSINDRALS LLQRTRATKNLFPRREERRR YESSKSDLDIETDYEDQAGN LEIETENEEEAEMETEVPAP VRTHSYALDRYVRQKRREKQ RKQSLKRVEKKYTPSELALY EIRKYQRSTDLLISKIPFAR LVKEVTDEFTTKDQDLRWQS MAIMALQEASEAYLVGLLEH TNLLALHAKRITIMKKDMQL ARRIRGQFI

Data sets:
Data typeCount
13C chemical shifts4
15N chemical shifts65
1H chemical shifts65

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cse41

Entities:

Entity 1, Cse4 229 residues - Formula weight is not available

1   METSERSERLYSGLNGLNTRPVALSERSER
2   ALAILEGLNSERASPSERSERGLYARGSER
3   LEUSERASNVALASNARGLEUALAGLYASP
4   GLNGLNSERILEASNASPARGALALEUSER
5   LEULEUGLNARGTHRARGALATHRLYSASN
6   LEUPHEPROARGARGGLUGLUARGARGARG
7   TYRGLUSERSERLYSSERASPLEUASPILE
8   GLUTHRASPTYRGLUASPGLNALAGLYASN
9   LEUGLUILEGLUTHRGLUASNGLUGLUGLU
10   ALAGLUMETGLUTHRGLUVALPROALAPRO
11   VALARGTHRHISSERTYRALALEUASPARG
12   TYRVALARGGLNLYSARGARGGLULYSGLN
13   ARGLYSGLNSERLEULYSARGVALGLULYS
14   LYSTYRTHRPROSERGLULEUALALEUTYR
15   GLUILEARGLYSTYRGLNARGSERTHRASP
16   LEULEUILESERLYSILEPROPHEALAARG
17   LEUVALLYSGLUVALTHRASPGLUPHETHR
18   THRLYSASPGLNASPLEUARGTRPGLNSER
19   METALAILEMETALALEUGLNGLUALASER
20   GLUALATYRLEUVALGLYLEULEUGLUHIS
21   THRASNLEULEUALALEUHISALALYSARG
22   ILETHRILEMETLYSLYSASPMETGLNLEU
23   ALAARGARGILEARGGLYGLNPHEILE

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks