BMRB Entry 27592

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27592

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for BM13851 strain of P[8] VP8* rotavirus
Deposition date:
2018-08-24
Original release date:
2020-03-10
Authors:
Kennedy, Michael; Xu, Shenyuan; Jiang, Xi; Liu, Yang
Citation:

Citation: Xu, Shenyuan; Ahmed, Luay; Stuckert, Michael; McGinnis, Kristen; Liu, Yang; Tan, Ming; Huang, Pengwei; Zhong, Weiming; Zhao, Dandan; Jiang, Xi; Kennedy, Michael. "Molecular basis of P[II] major human rotavirus VP8* domain recognition of histo-blood group antigens"  PLOS Pathogens 16, e1008386-e1008386 (2020).
PubMed: 32208455

Assembly members:

Assembly members:
BM13851, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rotavirus   Taxonomy ID: 10912   Superkingdom: Viruses   Kingdom: not available   Genus/species: Rotavirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BM13851: GSILDGPYQPTTFTPPTDYW ILINSNTNGVVYESTNNSDF WTAVIAVEPHVNPVDRQYNV FGENKQFNVRNDSDKWKFLE MFRGSSQNDFYNRRTLTSDT RLVGILKYGGRIWTFHGETP RATTDSSNTANLNGISITIH SEFYIIPRSQESKCNEYINN GL

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts148
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BM13851 strain of P[8] VP8* rotavirus1

Entities:

Entity 1, BM13851 strain of P[8] VP8* rotavirus 162 residues - Formula weight is not available

1   GLYSERILELEUASPGLYPROTYRGLNPRO
2   THRTHRPHETHRPROPROTHRASPTYRTRP
3   ILELEUILEASNSERASNTHRASNGLYVAL
4   VALTYRGLUSERTHRASNASNSERASPPHE
5   TRPTHRALAVALILEALAVALGLUPROHIS
6   VALASNPROVALASPARGGLNTYRASNVAL
7   PHEGLYGLUASNLYSGLNPHEASNVALARG
8   ASNASPSERASPLYSTRPLYSPHELEUGLU
9   METPHEARGGLYSERSERGLNASNASPPHE
10   TYRASNARGARGTHRLEUTHRSERASPTHR
11   ARGLEUVALGLYILELEULYSTYRGLYGLY
12   ARGILETRPTHRPHEHISGLYGLUTHRPRO
13   ARGALATHRTHRASPSERSERASNTHRALA
14   ASNLEUASNGLYILESERILETHRILEHIS
15   SERGLUPHETYRILEILEPROARGSERGLN
16   GLUSERLYSCYSASNGLUTYRILEASNASN
17   GLYLEU

Samples:

sample_1: P[8] VP8* polypeptide, [U-13C; U-15N], 0.6 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM

sample_conditions_1: ionic strength: 200 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks