BMRB Entry 27583

Name: The structure of the Pro-domain of mouse proNGF in contact with the NGF domain
Published: 2018-09-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27583

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The structure of the Pro-domain of mouse proNGF in contact with the NGF domain

Deposition date:

2018-08-21

Original release date:

2018-09-21

Authors:

Yan, Robert; Yalinca, Havva; Paoletti, Francesca; Gobbo, Francesco; Marchetti, Laura; Kuzmanic, Antonija; Lamba, Doriano; Gervasio, Francesco; Konarev, Petr; Cattaneo, Antonino; Pastore, Annalisa

Citation:

Citation: Yan, Robert; Yalinca, Havva; Paoletti, Francesca; Gobbo, Francesco; Marchetti, Laura; Kuzmanic, Antonija; Lamba, Doriano; Gervasio, Francesco; Konarev, Petr; Cattaneo, Antonino; Pastore, Annalisa. "The structure of the Pro-domain of mouse proNGF in contact with the NGF domain" Structure 27, 78-89 (2019).
PubMed: 30393051

Assembly members:

Assembly members:
NGF_pro-domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NGF_pro-domain: MEPYTDSNVPEGDSVPEAHW TKLQHSLDTALRRARSAPTA PIAARVTGQTRNITVDPRLF KKRRLHSPRVLFSTQPPPTS SDTLDLDFQAHGTIPFNRTH RSKR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	89
1H chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NGF Pro-domain	1

Entities:

Entity 1, NGF Pro-domain 104 residues - Formula weight is not available

1

MET

GLU

PRO

TYR

THR

ASP

SER

ASN

VAL

PRO

2

GLU

GLY

ASP

SER

VAL

PRO

GLU

ALA

HIS

TRP

3

THR

LYS

LEU

GLN

HIS

SER

LEU

ASP

THR

ALA

4

LEU

ARG

ALA

ARG

SER

ALA

PRO

THR

ALA

5

PRO

ILE

ALA

ARG

VAL

THR

GLY

GLN

THR

6

ARG

ASN

ILE

THR

VAL

ASP

PRO

ARG

LEU

PHE

7

LYS

ARG

LEU

HIS

SER

PRO

ARG

VAL

8

LEU

PHE

SER

THR

GLN

PRO

THR

SER

9

SER

ASP

THR

LEU

ASP

LEU

ASP

PHE

GLN

ALA

10

HIS

GLY

THR

ILE

PRO

PHE

ASN

ARG

THR

HIS

11

ARG

SER

LYS

ARG

Samples:

sample_1: NGF pro-domain, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 50 mM; NaCl 50 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMR-STAR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks