BMRB Entry 27577

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27577

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Title:
Resonance assignments for Ras-related C3 botulinum toxin substrate 1 bound to GDP and Mg2+
Deposition date:
2018-08-13
Original release date:
2019-03-14
Authors:
Toyama, Yuki; Kontani, Kenji; Katada, Toshiaki; Shimada, Ichio
Citation:

Citation: Toyama, Yuki; Kontani, Kenji; Katada, Toshiaki; Shimada, Ichio. "Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR"  Sci. Adv. 5, 8945-8945 (2019).

Assembly members:

Assembly members:
Ras-related_C3_botulinum_toxin_substrate_1, polymer, 192 residues, Formula weight is not available
entity_MG, non-polymer, 24.305 Da.
entity_GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ras-related_C3_botulinum_toxin_substrate_1: GPLGSPEFMQAIKCVVVGDG AVGKTCLLISYTTNAFPGEY IPTVFDNYSANVMVDGKPVN LGLWDTAGQEDYDRLRPLSY PQTDVFLICFSLVSPASFEN VRAKWYPEVRHHCPNTPIIL VGTKLDLRDDKDTIEKLKEK KLTPITYPQGLAMAKEIGAV KYLECSALTQRGLKTVFDEA IRAVLCPPPVKK

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts153
1H chemical shifts390

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rac11
2Mg2
3GDP3

Entities:

Entity 1, Rac1 192 residues - Formula weight is not available

Residues 1-8 represent a cloning artifact.

1   GLYPROLEUGLYSERPROGLUPHEMETGLN
2   ALAILELYSCYSVALVALVALGLYASPGLY
3   ALAVALGLYLYSTHRCYSLEULEUILESER
4   TYRTHRTHRASNALAPHEPROGLYGLUTYR
5   ILEPROTHRVALPHEASPASNTYRSERALA
6   ASNVALMETVALASPGLYLYSPROVALASN
7   LEUGLYLEUTRPASPTHRALAGLYGLNGLU
8   ASPTYRASPARGLEUARGPROLEUSERTYR
9   PROGLNTHRASPVALPHELEUILECYSPHE
10   SERLEUVALSERPROALASERPHEGLUASN
11   VALARGALALYSTRPTYRPROGLUVALARG
12   HISHISCYSPROASNTHRPROILEILELEU
13   VALGLYTHRLYSLEUASPLEUARGASPASP
14   LYSASPTHRILEGLULYSLEULYSGLULYS
15   LYSLEUTHRPROILETHRTYRPROGLNGLY
16   LEUALAMETALALYSGLUILEGLYALAVAL
17   LYSTYRLEUGLUCYSSERALALEUTHRGLN
18   ARGGLYLEULYSTHRVALPHEASPGLUALA
19   ILEARGALAVALLEUCYSPROPROPROVAL
20   LYSLYS

Entity 2, Mg - Mg - 24.305 Da.

1   MG

Entity 3, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Related Database Links:

UNP P63000
AlphaFold Q9BTB4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks