BMRB Entry 27565

Name: Transmembrane protein 106B (TEM106B)
Published: 2018-11-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27565

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Transmembrane protein 106B (TEM106B)

Deposition date:

2018-07-30

Original release date:

2018-11-15

Authors:

Lim, Liang Zhong; Kang, Jian; Song, Jianxing

Citation:

Citation: Kang, Jian; Lim, Liang Zhong; Song, Jianxing. "TMEM106B, a risk factor for FTLD and aging, has an intrinsically disordered cytoplasmic domain" PLoS One 13, e0205856-e0205856 (2018).
PubMed: 30332472

Assembly members:

Assembly members:
TMEM106B, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TMEM106B: MGKSLSHLPLHSSKEDAYDG VTSENMRNGLVNSEVHNEDG RNGDVSQFPYVEFTGRDSVT CPTCQGTGRIPRGQENQLVA LIPYSDQRLRPRRLEHHHHH H

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	179
15N chemical shifts	88
1H chemical shifts	307

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TMEM106B	1

Entities:

Entity 1, TMEM106B 101 residues - Formula weight is not available

1

MET

GLY

LYS

SER

LEU

SER

HIS

LEU

PRO

LEU

2

HIS

SER

LYS

GLU

ASP

ALA

TYR

ASP

GLY

3

VAL

THR

SER

GLU

ASN

MET

ARG

ASN

GLY

LEU

4

VAL

ASN

SER

GLU

VAL

HIS

ASN

GLU

ASP

GLY

5

ARG

ASN

GLY

ASP

VAL

SER

GLN

PHE

PRO

TYR

6

VAL

GLU

PHE

THR

GLY

ARG

ASP

SER

VAL

THR

7

CYS

PRO

THR

CYS

GLN

GLY

THR

GLY

ARG

ILE

8

PRO

ARG

GLY

GLN

GLU

ASN

GLN

LEU

VAL

ALA

9

LEU

ILE

PRO

TYR

SER

ASP

GLN

ARG

LEU

ARG

10

PRO

ARG

LEU

GLU

HIS

11

HIS

Samples:

sample_1: D2O 5%; TMEM106B, [U-100% 13C; U-100% 15N], 0.2 ± 0.005 mM; potassium phosphate 5 mM

sample_conditions_1: ionic strength: 0.02 Not defined; pH: 6.5; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, CCPN, Cornilescu, Delaglio and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, peak picking, processing

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks