BMRB Entry 27558
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27558
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NMR-STAR v3 text file.
XML gzip file.
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Citation: De March, Matteo; Barrera-Vilarmau, Susana; Crespan, Emmanuele; Mentegari, Elisa; Merino, Nekane; Gonzalez-Magana, Amaia; Romano-Moreno, Miguel; Maga, Giovanni; Crehuet, Ramon; Onesti, Silvia; Blanco, Francisco; De Biasio, Alfredo. "p15PAF binding to PCNA modulates the DNA sliding surface." Nucleic Acids Res. 46, 9816-9828 (2018).
PubMed: 30102405
Assembly members:
PCNA, polymer, 264 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
PCNA: GPHMFEARLVQGSILKKVLE
ALKDLINEACWDISSSGVNL
QSMDSSHVSLVQLTLRSEGF
DTYRCDRNLAMGVNLTSMSK
ILKCAGNEDIITLRAEDNAD
TLALVFEAPNQEKVSDYEMK
LMDLDVEQLGIPEQEYSCVV
KMPSGEFARICRDLSHIGDA
VVISCAKDGVKFSASGELGN
GNIKLSQTSNVDKEEEAVTI
EMNEPVQLTFALRYLNFFTK
ATPLSSTVTLSMSADVPLVV
EYKIADMGHLKYYLAPKIED
EEGS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 151 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PCNA | 1 |
Entities:
Entity 1, PCNA 264 residues - Formula weight is not available
| 1 | GLY | PRO | HIS | MET | PHE | GLU | ALA | ARG | LEU | VAL | ||||
| 2 | GLN | GLY | SER | ILE | LEU | LYS | LYS | VAL | LEU | GLU | ||||
| 3 | ALA | LEU | LYS | ASP | LEU | ILE | ASN | GLU | ALA | CYS | ||||
| 4 | TRP | ASP | ILE | SER | SER | SER | GLY | VAL | ASN | LEU | ||||
| 5 | GLN | SER | MET | ASP | SER | SER | HIS | VAL | SER | LEU | ||||
| 6 | VAL | GLN | LEU | THR | LEU | ARG | SER | GLU | GLY | PHE | ||||
| 7 | ASP | THR | TYR | ARG | CYS | ASP | ARG | ASN | LEU | ALA | ||||
| 8 | MET | GLY | VAL | ASN | LEU | THR | SER | MET | SER | LYS | ||||
| 9 | ILE | LEU | LYS | CYS | ALA | GLY | ASN | GLU | ASP | ILE | ||||
| 10 | ILE | THR | LEU | ARG | ALA | GLU | ASP | ASN | ALA | ASP | ||||
| 11 | THR | LEU | ALA | LEU | VAL | PHE | GLU | ALA | PRO | ASN | ||||
| 12 | GLN | GLU | LYS | VAL | SER | ASP | TYR | GLU | MET | LYS | ||||
| 13 | LEU | MET | ASP | LEU | ASP | VAL | GLU | GLN | LEU | GLY | ||||
| 14 | ILE | PRO | GLU | GLN | GLU | TYR | SER | CYS | VAL | VAL | ||||
| 15 | LYS | MET | PRO | SER | GLY | GLU | PHE | ALA | ARG | ILE | ||||
| 16 | CYS | ARG | ASP | LEU | SER | HIS | ILE | GLY | ASP | ALA | ||||
| 17 | VAL | VAL | ILE | SER | CYS | ALA | LYS | ASP | GLY | VAL | ||||
| 18 | LYS | PHE | SER | ALA | SER | GLY | GLU | LEU | GLY | ASN | ||||
| 19 | GLY | ASN | ILE | LYS | LEU | SER | GLN | THR | SER | ASN | ||||
| 20 | VAL | ASP | LYS | GLU | GLU | GLU | ALA | VAL | THR | ILE | ||||
| 21 | GLU | MET | ASN | GLU | PRO | VAL | GLN | LEU | THR | PHE | ||||
| 22 | ALA | LEU | ARG | TYR | LEU | ASN | PHE | PHE | THR | LYS | ||||
| 23 | ALA | THR | PRO | LEU | SER | SER | THR | VAL | THR | LEU | ||||
| 24 | SER | MET | SER | ALA | ASP | VAL | PRO | LEU | VAL | VAL | ||||
| 25 | GLU | TYR | LYS | ILE | ALA | ASP | MET | GLY | HIS | LEU | ||||
| 26 | LYS | TYR | TYR | LEU | ALA | PRO | LYS | ILE | GLU | ASP | ||||
| 27 | GLU | GLU | GLY | SER |
Samples:
sample_1: PCNA 0.1 mM; P15(50-77) 0.64 mM; DNA duplex 0.1 mM; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM
sample_conditions_1: ionic strength: 0.06 M; pH: 7.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-1H-15N-TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks