BMRB Entry 27553

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27553

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Title:
1H, 15N, and 13C resonance assignments of the intrinsically disordered SH4 and Unique domains of p59Hck
Deposition date:
2018-07-23
Original release date:
2018-11-15
Authors:
Pond, Matthew; Roux, Benoit
Citation:

Citation: Pond, Matthew; Blachowicz, Lydia; Roux, Benoit. "1H, 15N, and 13C resonance assignments of the intrinsically disordered SH4 and Unique domains of Hck"  Biomol. NMR Assignments 13, 71-74 (2019).
PubMed: 30284668

Assembly members:

Assembly members:
Hck, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hck: GCMKSKFLQVGGNTFSKTET SASPHCPVYVPDPTSTIKPG PNSHNSNTPGIREAGSE

Data sets:
Data typeCount
13C chemical shifts165
15N chemical shifts49
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hck1

Entities:

Entity 1, Hck 57 residues - Formula weight is not available

1   GLYCYSMETLYSSERLYSPHELEUGLNVAL
2   GLYGLYASNTHRPHESERLYSTHRGLUTHR
3   SERALASERPROHISCYSPROVALTYRVAL
4   PROASPPROTHRSERTHRILELYSPROGLY
5   PROASNSERHISASNSERASNTHRPROGLY
6   ILEARGGLUALAGLYSERGLU

Samples:

sample_1: Hck, [U-13C; U-15N], 700 uM; D2O, [U-2H], 5%; TCEP 5 mM; sodium acetate 50 mM

sample_2: Hck, [U-15N], 550 uM; D2O, [U-2H], 10%; sodium acetate 50 mM; TCEP 5 mM

sample_conditions_1: ionic strength: 55 mM; pH: 4.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

MddNMR, V. Orekhov, V. Jaravine, M. Mayzel, K. Kazimierczuk - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks