BMRB Entry 27547

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27547
MolProbity Validation Chart

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Title:
Backbone chemical shift assignments of H2A in H2A-H2B dimer
Deposition date:
2018-07-17
Original release date:
2019-07-26
Authors:
van Ingen, Hugo; Eerland, Jelmer; Horn, Velten; Svatos, Alma; Zhang, Heyi
Citation:

Citation: Horn, Velten; Uckelmann, Michael; Zhang, Heyi; Eerland, Jelmer; Aarsman, Ivette; Le Paige, Ulric; Davidovich, Chen; Sixma, Titia; van Ingen, Hugo. "Structural basis of specific H2A K13/K15 ubiquitination by RNF168."  Nat. Commun. 10, 1751-1751 (2019).
PubMed: 30988309

Assembly members:

Assembly members:
H2A, polymer, 123 residues, Formula weight is not available
H2B, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H2A: SGRGKGGKVKGKAKSRSNRA GLQFPVGRIHRLLRKGNYAE RVGAGAPVYLAAVMEYLAAE VLELAGNAARDNKKTRIIPR HLQLAIRNDEELNKLLSGVT IAQGGVLPNIQAVLLPKKTE KKA
H2B: IPPKTSGKAAKKAGKAQKNI TKTDKKKKRKRKESYAIYIY KVLKQVHPDTGISSKAMSIM NSFVNDIFERIAAEASRLAH YNKRSTITSREIQTAVRLLL PGELAKHAVSEGTKAVTKYT SSK

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1H2A, subunit 11
2H2B, subunit 22

Entities:

Entity 1, H2A, subunit 1 123 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSVALLYS
2   GLYLYSALALYSSERARGSERASNARGALA
3   GLYLEUGLNPHEPROVALGLYARGILEHIS
4   ARGLEULEUARGLYSGLYASNTYRALAGLU
5   ARGVALGLYALAGLYALAPROVALTYRLEU
6   ALAALAVALMETGLUTYRLEUALAALAGLU
7   VALLEUGLULEUALAGLYASNALAALAARG
8   ASPASNLYSLYSTHRARGILEILEPROARG
9   HISLEUGLNLEUALAILEARGASNASPGLU
10   GLULEUASNLYSLEULEUSERGLYVALTHR
11   ILEALAGLNGLYGLYVALLEUPROASNILE
12   GLNALAVALLEULEUPROLYSLYSTHRGLU
13   LYSLYSALA

Entity 2, H2B, subunit 2 123 residues - Formula weight is not available

1   ILEPROPROLYSTHRSERGLYLYSALAALA
2   LYSLYSALAGLYLYSALAGLNLYSASNILE
3   THRLYSTHRASPLYSLYSLYSLYSARGLYS
4   ARGLYSGLUSERTYRALAILETYRILETYR
5   LYSVALLEULYSGLNVALHISPROASPTHR
6   GLYILESERSERLYSALAMETSERILEMET
7   ASNSERPHEVALASNASPILEPHEGLUARG
8   ILEALAALAGLUALASERARGLEUALAHIS
9   TYRASNLYSARGSERTHRILETHRSERARG
10   GLUILEGLNTHRALAVALARGLEULEULEU
11   PROGLYGLULEUALALYSHISALAVALSER
12   GLUGLYTHRLYSALAVALTHRLYSTYRTHR
13   SERSERLYS

Samples:

sample_1: H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 0.3 mM; H2B 0.3 mM; MES 20 mM; sodium chloride 90 mM

sample_conditions_1: ionic strength: 100 mM; pH: 5.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks