BMRB Entry 27546

Name: Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment
Published: 2018-07-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27546

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment

Deposition date:

2018-07-13

Original release date:

2018-07-19

Authors:

Bernard, David; Doucet, Nicolas

Citation:

Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment" .

Assembly members:

Assembly members:
AtEDN, polymer, 131 residues, 14857 Da.

Natural source:

Natural source: Common Name: douroucouli Taxonomy ID: 9505 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Aotus trivirgatus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress 414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AtEDN: APQKFTRAQWFSIQHIQTTP LRCTNAMRAINKYQHRCKNQ NTFLHTTFAAVVNVCGNTNI TCPRNASLNNCHHSRVQVPL TYCNLTGPPTITNCVYSSTQ ANMFYVVACDNRDQRDPPQY PVVPVHLDTTI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	254
15N chemical shifts	119
1H chemical shifts	119

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Aotus trivirgatus EDN	1

Entities:

Entity 1, Aotus trivirgatus EDN 131 residues - 14857 Da.

1

ALA

PRO

GLN

LYS

PHE

THR

ARG

ALA

GLN

TRP

2

PHE

SER

ILE

GLN

HIS

ILE

GLN

THR

PRO

3

LEU

ARG

CYS

THR

ASN

ALA

MET

ARG

ALA

ILE

4

ASN

LYS

TYR

GLN

HIS

ARG

CYS

LYS

ASN

GLN

5

ASN

THR

PHE

LEU

HIS

THR

PHE

ALA

6

VAL

ASN

VAL

CYS

GLY

ASN

THR

ASN

ILE

7

THR

CYS

PRO

ARG

ASN

ALA

SER

LEU

ASN

8

CYS

HIS

SER

ARG

VAL

GLN

VAL

PRO

LEU

9

THR

TYR

CYS

ASN

LEU

THR

GLY

PRO

THR

10

ILE

THR

ASN

CYS

VAL

TYR

SER

THR

GLN

11

ALA

ASN

MET

PHE

TYR

VAL

ALA

CYS

ASP

12

ASN

ARG

ASP

GLN

ARG

ASP

PRO

GLN

TYR

13

PRO

VAL

PRO

VAL

HIS

LEU

ASP

THR

14

ILE

Samples:

sample_1: AtEDN, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr v2.4, CCPN - peak picking

NMR spectrometers:

Bruker Avance 600 MHz

UNP	O18937
EMBL	AAB71338.1
AlphaFold	O18937