BMRB Entry 27544

Name: Pongo pygmaeus Eosinophil Cationic Protein backbone assignment
Published: 2018-07-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27544

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Pongo pygmaeus Eosinophil Cationic Protein backbone assignment

Deposition date:

2018-07-13

Original release date:

2018-07-19

Authors:

Bernard, David; Doucet, Nicolas

Citation:

Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Pongo pygmaeus Eosinophil Cationic Protein backbone assignment" .

Assembly members:

Assembly members:
PpECP, polymer, 133 residues, 15503 Da.

Natural source:

Natural source: Common Name: Bornean orangutan Taxonomy ID: 9600 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Pongo pygmaeus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress 414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PpECP: KPRQFTRAQWFAIQHVSLNP PQCTTAMRVINNYQRRCKDQ NTFLRTTFANVVNVCGNPNI TCPRNRTLHNCHRSRFQVPL LHCNLTNPGAQNISNCKYAD RTERRFYVVACDNRDPRDSP RYPVVPVHLDTTI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	259
15N chemical shifts	122
1H chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pongo pygmaeus ECP	1

Entities:

Entity 1, Pongo pygmaeus ECP 133 residues - 15503 Da.

1

LYS

PRO

ARG

GLN

PHE

THR

ARG

ALA

GLN

TRP

2

PHE

ALA

ILE

GLN

HIS

VAL

SER

LEU

ASN

PRO

3

PRO

GLN

CYS

THR

ALA

MET

ARG

VAL

ILE

4

ASN

TYR

GLN

ARG

CYS

LYS

ASP

GLN

5

ASN

THR

PHE

LEU

ARG

THR

PHE

ALA

ASN

6

VAL

ASN

VAL

CYS

GLY

ASN

PRO

ASN

ILE

7

THR

CYS

PRO

ARG

ASN

ARG

THR

LEU

HIS

ASN

8

CYS

HIS

ARG

SER

ARG

PHE

GLN

VAL

PRO

LEU

9

LEU

HIS

CYS

ASN

LEU

THR

ASN

PRO

GLY

ALA

10

GLN

ASN

ILE

SER

ASN

CYS

LYS

TYR

ALA

ASP

11

ARG

THR

GLU

ARG

PHE

TYR

VAL

ALA

12

CYS

ASP

ASN

ARG

ASP

PRO

ARG

ASP

SER

PRO

13

ARG

TYR

PRO

VAL

PRO

VAL

HIS

LEU

ASP

14

THR

ILE

Samples:

sample_1: PpECP, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr v2.4, CCPN - peak picking

NMR spectrometers:

Varian INOVA 500 MHz

UNP	P47781
EMBL	AAC50147.1
AlphaFold	P47781