BMRB Entry 27542

Name: Macaca fascicularis Eosinophil Cationic Protein backbone assignment
Published: 2018-07-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27542

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Macaca fascicularis Eosinophil Cationic Protein backbone assignment

Deposition date:

2018-07-12

Original release date:

2018-07-19

Authors:

Bernard, David; Doucet, Nicolas

Citation:

Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Macaca fascicularis Eosinophil Cationic Protein backbone assignment" .

Assembly members:

Assembly members:
MfECP, polymer, 133 residues, 15745 Da.

Natural source:

Natural source: Common Name: crab-eating macaque Taxonomy ID: 9541 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macaca fascicularis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress 414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MfECP: RPPQFTKAQWFAIQHINVNP PRCTIAMRVINNYQRRCKNQ NTFLRTTFAYTANVCRNERI RCPRNRTLHNCHRSRYRVPL LHCDLINPGAQNISTCRYAD RPGRRFYVVACESRDPRDSP RYPVVPVHLDTTI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	257
15N chemical shifts	131
1H chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Macaca fascicularis ECP	1

Entities:

Entity 1, Macaca fascicularis ECP 133 residues - 15745 Da.

1

ARG

PRO

GLN

PHE

THR

LYS

ALA

GLN

TRP

2

PHE

ALA

ILE

GLN

HIS

ILE

ASN

VAL

ASN

PRO

3

PRO

ARG

CYS

THR

ILE

ALA

MET

ARG

VAL

ILE

4

ASN

TYR

GLN

ARG

CYS

LYS

ASN

GLN

5

ASN

THR

PHE

LEU

ARG

THR

PHE

ALA

TYR

6

THR

ALA

ASN

VAL

CYS

ARG

ASN

GLU

ARG

ILE

7

ARG

CYS

PRO

ARG

ASN

ARG

THR

LEU

HIS

ASN

8

CYS

HIS

ARG

SER

ARG

TYR

ARG

VAL

PRO

LEU

9

LEU

HIS

CYS

ASP

LEU

ILE

ASN

PRO

GLY

ALA

10

GLN

ASN

ILE

SER

THR

CYS

ARG

TYR

ALA

ASP

11

ARG

PRO

GLY

ARG

PHE

TYR

VAL

ALA

12

CYS

GLU

SER

ARG

ASP

PRO

ARG

ASP

SER

PRO

13

ARG

TYR

PRO

VAL

PRO

VAL

HIS

LEU

ASP

14

THR

ILE

Samples:

sample_1: MfECP, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM

sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr, CCPN - peak picking

NMR spectrometers:

Varian INOVA 500 MHz

UNP	P47779
EMBL	U24098.1
AlphaFold	P47779