BMRB Entry 27530

Name: apoCaM bound to Cav1.2 IQ
Published: 2019-09-17

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PDB ID: 6ctb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27530
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

apoCaM bound to Cav1.2 IQ

Deposition date:

2018-06-28

Original release date:

2019-09-17

Authors:

Turner, Matthew; Ames, James; Anderson, David

Citation:

Citation: Turner, Matthew; Ames, James; Anderson, David. "ApoCaM bound to Cav1.2 IQ" Cell ., .-..

Assembly members:

Assembly members:
apoCaM, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apoCaM: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARKMKDT DSEEEIREAFRVFDKDGNGY ISAAELRHVMTNLGEKLTDE EVDEMIREADIDGDGQVNYE EFVQMMTAK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	115
1H chemical shifts	115

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apoCaM	1

Entities:

Entity 1, apoCaM 149 residues - Formula weight is not available

1

MET

ALA

ASP

GLN

LEU

THR

GLU

GLN

ILE

2

ALA

GLU

PHE

LYS

GLU

ALA

PHE

SER

LEU

PHE

3

ASP

LYS

ASP

GLY

ASP

GLY

THR

ILE

THR

4

LYS

GLU

LEU

GLY

THR

VAL

MET

ARG

SER

LEU

5

GLY

GLN

ASN

PRO

THR

GLU

ALA

GLU

LEU

GLN

6

ASP

MET

ILE

ASN

GLU

VAL

ASP

ALA

ASP

GLY

7

ASN

GLY

THR

ILE

ASP

PHE

PRO

GLU

PHE

LEU

8

THR

MET

ALA

ARG

LYS

MET

LYS

ASP

THR

9

ASP

SER

GLU

ILE

ARG

GLU

ALA

PHE

10

ARG

VAL

PHE

ASP

LYS

ASP

GLY

ASN

GLY

TYR

11

ILE

SER

ALA

GLU

LEU

ARG

HIS

VAL

MET

12

THR

ASN

LEU

GLY

GLU

LYS

LEU

THR

ASP

GLU

13

GLU

VAL

ASP

GLU

MET

ILE

ARG

GLU

ALA

ASP

14

ILE

ASP

GLY

ASP

GLY

GLN

VAL

ASN

TYR

GLU

15

GLU

PHE

VAL

GLN

MET

THR

ALA

LYS

Samples:

sample_1: apoCaM, [U-100% 15N], 350 uM; apoCaM, [U-100% 13C; U-100% 15N], 350 uM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.3; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks