BMRB Entry 27524

Name: Sequence specific 1H, 13C and 15N resonance assignments of the C-terminal domain of human Gamma S-Crystallin
Published: 2018-06-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27524

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence specific 1H, 13C and 15N resonance assignments of the C-terminal domain of human Gamma S-Crystallin

Deposition date:

2018-06-22

Original release date:

2018-06-25

Authors:

Khandekar Bari, Jishan; Sharma, Shrikant; Chary, Kandala V.R.

Citation:

Citation: Khandekar Bari, Jishan; Sharma, Shrikant; Chary, Kandala V.R.. "Sequence specific 1H, 13C and 15N resonance assignments of the C-terminal domain of human Gamma S-crystallin" Biomol. NMR Assignments 13, 43-47 (2019).
PubMed: 30232732

Assembly members:

Assembly members:
C_terminal_domain_of_Human_Gamma_S_Crystallin, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C_terminal_domain_of_Human_Gamma_S_Crystallin: GGQYKIQIFEKGDFSGQMYE TTEDCPSIMEQFHMREIHSC KVLEGVWIFYELPNYRGRQY LLDKKEYRKPIDWGAASPAV QSFRRIVE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	323
15N chemical shifts	89
1H chemical shifts	595

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C terminal domain of Human Gamma S Crystallin	1

Entities:

Entity 1, C terminal domain of Human Gamma S Crystallin 88 residues - Formula weight is not available

1

GLY

GLN

TYR

LYS

ILE

GLN

ILE

PHE

GLU

2

LYS

GLY

ASP

PHE

SER

GLY

GLN

MET

TYR

GLU

3

THR

GLU

ASP

CYS

PRO

SER

ILE

MET

GLU

4

GLN

PHE

HIS

MET

ARG

GLU

ILE

HIS

SER

CYS

5

LYS

VAL

LEU

GLU

GLY

VAL

TRP

ILE

PHE

TYR

6

GLU

LEU

PRO

ASN

TYR

ARG

GLY

ARG

GLN

TYR

7

LEU

ASP

LYS

GLU

TYR

ARG

LYS

PRO

8

ILE

ASP

TRP

GLY

ALA

SER

PRO

ALA

VAL

9

GLN

SER

PHE

ARG

ILE

VAL

GLU

Samples:

sample_1: C terminal domain of Human Gamma S Crystallin, [U-99% 13C; U-99% 15N], 1.5 mM; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks