BMRB Entry 27517

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for HTLV-1 HBZ 3-56
Published: 2020-09-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27517

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for HTLV-1 HBZ 3-56

Deposition date:

2018-06-11

Original release date:

2020-09-26

Authors:

Yang, Ke; Stanfield, Robyn L.; Martinez-Yamout, Maria; Dyson, H. Jane; Wilson, Ian A.; Wright, Peter E.

Citation:

Citation: Yang, Ke; Stanfield, Robyn; Martinez-Yamout, Maria; Dyson, H Jane; Wilson, Ian; Wright, Peter. "Structural basis for cooperative regulation of KIX-mediated transcription pathways by the HTLV-1 HBZ activation domain" Proc. Natl. Acad. Sci. U.S.A. 115, 10040-10045 (2018).
PubMed: 30232260

Assembly members:

Assembly members:
HBZ_activation_domain, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human T-cell leukemia virus Taxonomy ID: 11908 Superkingdom: Viruses Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HBZ_activation_domain: GSHMASGLFRALPVSAPEDL LVEELVDGLLSLEEELKDKE EEKAVLDGLLSLEEESRG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	104
15N chemical shifts	52
1H chemical shifts	52

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HBZ activation domain	1

Entities:

Entity 1, HBZ activation domain 58 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

SER

GLY

LEU

PHE

ARG

2

ALA

LEU

PRO

VAL

SER

ALA

PRO

GLU

ASP

LEU

3

LEU

VAL

GLU

LEU

VAL

ASP

GLY

LEU

4

SER

LEU

GLU

LEU

LYS

ASP

LYS

GLU

5

GLU

LYS

ALA

VAL

LEU

ASP

GLY

LEU

6

SER

LEU

GLU

SER

ARG

GLY

Samples:

sample_1: TRIS 50 mM; sodium chloride 50 mM; HBZ activation domain, [U-98% 13C; U-98% 15N], 600 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.80; pressure: 1 atm; temperature: 301 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks