BMRB Entry 27508

Name: Backbone resonance assignemnts of a Smurf2 HECT domain C-lobe mutant
Published: 2018-07-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27508

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone resonance assignemnts of a Smurf2 HECT domain C-lobe mutant

Deposition date:

2018-06-06

Original release date:

2018-07-12

Authors:

Jaeckl, Magnus; Stollmaier, Carsten; Stroh ker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke

Citation:

Citation: Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke. "Beta-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation" J. Mol. Biol. 430, 3218-3233 (2018).
PubMed: 29964046

Assembly members:

Assembly members:
clobe, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
clobe: GAMGKIDVNDWKVNTRLKHA TPDSNIVKWFWKAVEFFDEE RRARLLQFVTGSSRVPLQGF KALQGAAGPRLFTIHQIDAN TNNLPKAHTCFNRIDIPPYE SYEKLYEKLLTAIEETSGFA VE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	234
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	clobe	1

Entities:

Entity 1, clobe 122 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

LYS

ILE

ASP

VAL

ASN

ASP

2

TRP

LYS

VAL

ASN

THR

ARG

LEU

LYS

HIS

ALA

3

THR

PRO

ASP

SER

ASN

ILE

VAL

LYS

TRP

PHE

4

TRP

LYS

ALA

VAL

GLU

PHE

ASP

GLU

5

ARG

ALA

ARG

LEU

GLN

PHE

VAL

THR

6

GLY

SER

ARG

VAL

PRO

LEU

GLN

GLY

PHE

7

LYS

ALA

LEU

GLN

GLY

ALA

GLY

PRO

ARG

8

LEU

PHE

THR

ILE

HIS

GLN

ILE

ASP

ALA

ASN

9

THR

ASN

LEU

PRO

LYS

ALA

HIS

THR

CYS

10

PHE

ASN

ARG

ILE

ASP

ILE

PRO

TYR

GLU

11

SER

TYR

GLU

LYS

LEU

TYR

GLU

LYS

LEU

12

THR

ALA

ILE

GLU

THR

SER

GLY

PHE

ALA

13

VAL

GLU

Samples:

sample_1: clobe, [U-100% 13C; U-100% 15N], 2.1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-99% 2H], 10 % w/v

sample_conditions_1: ionic strength: 0.2 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks