BMRB Entry 27488

Title:
ASK1-TBD
Deposition date:
2018-05-22
Original release date:
2020-10-06
Authors:
Psenakova, Katarina; Veverka, Vaclav
Citation:

Citation: Psenakova, Katarina; Hexnerova, Rozalie; Srb, Pavel; Obsilova, Veronika; Veverka, Vaclav; Obsil, Tomas. "The redox-active site of thioredoxin is directly involved in apoptosis signal-regulating kinase 1 binding that is modulated by oxidative stress"  FEBS J. 287, 1626-1644 (2020).
PubMed: 31623019

Assembly members:

Assembly members:
ASK1-TBD, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: N/A

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts120
1H chemical shifts468

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASK1-TBD1

Entities:

Entity 1, ASK1-TBD 185 residues - Formula weight is not available

1   GLYALAMETGLYSERSERARGARGTHRTHR
2   VALALATYRVALILEASNGLUALASERGLN
3   GLYGLNLEUVALVALALAGLUSERGLUALA
4   LEUGLNSERLEUARGGLUALACYSGLUTHR
5   VALGLYALATHRLEUGLUTHRLEUHISPHE
6   GLYLYSLEUASPPHEGLYGLUTHRTHRVAL
7   LEUASPARGPHETYRASNALAASPILEALA
8   VALVALGLUMETSERASPALAPHEARGGLN
9   PROSERLEUPHETYRHISLEUGLYVALARG
10   GLUSERPHESERMETALAASNASNILEILE
11   LEUTYRCYSASPTHRASNSERASPSERLEU
12   GLNSERLEULYSGLUILEILECYSGLNLYS
13   ASNTHRMETCYSTHRGLYASNTYRTHRPHE
14   VALPROTYRMETILETHRPROHISASNLYS
15   VALTYRCYSCYSASPSERSERPHEMETLYS
16   GLYLEUTHRGLULEUMETGLNPROASNPHE
17   GLULEULEULEUGLYPROILECYSLEUPRO
18   LEUVALASPARGPHEILEGLNLEULEULYS
19   VALALAGLNALASER

Samples:

sample_1: ASK1-TBD, [U-100% 13C; U-100% 15N; U-80% 2H], 300 uM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks