BMRB Entry 27484

Name: Backbone assignment of KSHV ORF57 68-178
Published: 2018-09-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27484

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of KSHV ORF57 68-178

Deposition date:

2018-05-15

Original release date:

2018-09-24

Authors:

Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander

Citation:

Citation: Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander. "Overlapping motifs on the herpes viral proteins ICP27 and ORF57 mediate interactions with the mRNA export adaptors ALYREF and UIF" Sci. Rep. 8, 15005-15005 (2018).

Assembly members:

Assembly members:
ORF57, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human gammaherpesvirus 8 Taxonomy ID: 37296 Superkingdom: Viruses Kingdom: not available Genus/species: Rhadinovirus Human herpesvirus 8 type P

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ORF57: MGSSHHHHHHSSGLVPRGSH MGRIPKKSQGRSQLRSEIQF CSPLSRPRSPSPVNRYGKKI KFGTAGQNTRPPPEKRPRRR PRDRLQYGRTTRGGQCRAAP KRATRRPQVNCQRQDDDVRQ GVSDAVKKLRLP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ORF57 68-178	1

Entities:

Entity 1, ORF57 68-178 132 residues - Formula weight is not available

N-terminal tag introdued in cloning is: MGSSHHHHHHSSGLVPRGSHM followed by KSHV ORF57 residues 68-178.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

GLY

ARG

ILE

PRO

LYS

SER

GLN

GLY

4

ARG

SER

GLN

LEU

ARG

SER

GLU

ILE

GLN

PHE

5

CYS

SER

PRO

LEU

SER

ARG

PRO

ARG

SER

PRO

6

SER

PRO

VAL

ASN

ARG

TYR

GLY

LYS

ILE

7

LYS

PHE

GLY

THR

ALA

GLY

GLN

ASN

THR

ARG

8

PRO

GLU

LYS

ARG

PRO

ARG

9

PRO

ARG

ASP

ARG

LEU

GLN

TYR

GLY

ARG

THR

10

THR

ARG

GLY

GLN

CYS

ARG

ALA

PRO

11

LYS

ARG

ALA

THR

ARG

PRO

GLN

VAL

ASN

12

CYS

GLN

ARG

GLN

ASP

VAL

ARG

GLN

13

GLY

VAL

SER

ASP

ALA

VAL

LYS

LEU

ARG

14

LEU

PRO

Samples:

sample_1: ORF57, [U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 50 mM; L-Arginine 50 mM; L-Glutamate 50 mM; EDTA 1 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - processing

SPARKY v3.12, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker DRX 600 MHz

UNP	Q2HR75
AlphaFold	O40938